Effect of fibrin preparation on enhancement of fVIII activity
| Fibrinogen source . | EC50 (Fibrin) μg/mL . | FVIII activity enhancement VMax/V0 . | Comment . |
|---|---|---|---|
| HT HCI-0150R | 5 ± 2 | 2.4 ± 0.2 | Research-grade |
| ERL HFG 852 | 5 ± 2 | 3.2 ± 0.2 | Research-grade |
| ERL FIB2 | 2.4 ± 0.8 | 1.88 ± 0.07 | Plasminogen and VWF-depleted |
| ERL FIB3 | 1.7 ± 0.8 | 2.4 ± 0.2 | Plasminogen, VWF, and fibronectin-depleted |
| ERL P1 FIB | 0.5 ± 0.4 | 1.61 ± 0.07 | γ′ Fibrinogen-depleted |
| ERL P2 FIB | 0.6 ± 0.3 | 1.51 ± 0.04 | γ′ Fibrinogen-enriched |
| γA/γA | 3 ± 1 | 1.45 ± 0.05 | Recombinant (from CHO cells) |
| Fibrinogen source . | EC50 (Fibrin) μg/mL . | FVIII activity enhancement VMax/V0 . | Comment . |
|---|---|---|---|
| HT HCI-0150R | 5 ± 2 | 2.4 ± 0.2 | Research-grade |
| ERL HFG 852 | 5 ± 2 | 3.2 ± 0.2 | Research-grade |
| ERL FIB2 | 2.4 ± 0.8 | 1.88 ± 0.07 | Plasminogen and VWF-depleted |
| ERL FIB3 | 1.7 ± 0.8 | 2.4 ± 0.2 | Plasminogen, VWF, and fibronectin-depleted |
| ERL P1 FIB | 0.5 ± 0.4 | 1.61 ± 0.07 | γ′ Fibrinogen-depleted |
| ERL P2 FIB | 0.6 ± 0.3 | 1.51 ± 0.04 | γ′ Fibrinogen-enriched |
| γA/γA | 3 ± 1 | 1.45 ± 0.05 | Recombinant (from CHO cells) |
Parameters were evaluated in the presence of 0.5 nM fVIII, 0.5 nM factor IXa, 150 nM factor X, 20 μM extruded PS:PE:PC (4:20:76) vesicles, 0.2 unit/mL thrombin, and varying fibrin concentrations. Parameters were calculated from a curve fit of averaged data from 2 to 6 experiments each performed in duplicate.
CHO, Chinese hamster ovary.