Effects of UNC0638 on gene expression assessed by RNA-seq
| Categories and genes . | Log2 CPM . | Fold change . | FDR q-value . |
|---|---|---|---|
| Control gene | |||
| GAPDH | 11.861 | 0.871 | 0.261 |
| Globin genes | |||
| HBG1 | 9.567 | 3.276 | 1.63E-35 |
| HBG2 | 9.827 | 1.937 | 1.13E-11 |
| HBB | 14.472 | 0.683 | 0.0002 |
| HBD | 9.126 | 0.652 | 3.44E-05 |
| HBE1 | 2.485 | 1.017 | 0.950 |
| HBA1 | 10.982 | 0.844 | 0.154 |
| HBA2 | 10.630 | 0.853 | 0.187 |
| Erythroid transcription factors | |||
| GATA1 | 8.749 | 0.867 | 0.253 |
| GATA2 | 7.284 | 0.584 | 1.59E-07 |
| KLF1 | 10.036 | 0.920 | 0.540 |
| NFE2 | 9.094 | 0.998 | 0.995 |
| ZFPM1 | 8.600 | 0.866 | 0.246 |
| SOX6 | 5.867 | 1.856 | 6.41E-09 |
| γ-Globin repressors | |||
| BCL11A | 6.455 | 1.219 | 0.109 |
| MYB | 7.069 | 0.832 | 0.133 |
| Categories and genes . | Log2 CPM . | Fold change . | FDR q-value . |
|---|---|---|---|
| Control gene | |||
| GAPDH | 11.861 | 0.871 | 0.261 |
| Globin genes | |||
| HBG1 | 9.567 | 3.276 | 1.63E-35 |
| HBG2 | 9.827 | 1.937 | 1.13E-11 |
| HBB | 14.472 | 0.683 | 0.0002 |
| HBD | 9.126 | 0.652 | 3.44E-05 |
| HBE1 | 2.485 | 1.017 | 0.950 |
| HBA1 | 10.982 | 0.844 | 0.154 |
| HBA2 | 10.630 | 0.853 | 0.187 |
| Erythroid transcription factors | |||
| GATA1 | 8.749 | 0.867 | 0.253 |
| GATA2 | 7.284 | 0.584 | 1.59E-07 |
| KLF1 | 10.036 | 0.920 | 0.540 |
| NFE2 | 9.094 | 0.998 | 0.995 |
| ZFPM1 | 8.600 | 0.866 | 0.246 |
| SOX6 | 5.867 | 1.856 | 6.41E-09 |
| γ-Globin repressors | |||
| BCL11A | 6.455 | 1.219 | 0.109 |
| MYB | 7.069 | 0.832 | 0.133 |
RNA-seq analysis was performed in primary adult human erythroid cells at day 11 of erythroid differentiation and after 7 days of treatment with 0.25 μM UNC0638 or the vehicle control. Data represent the mean of 3 biological replicates for selected genes of interest.
CPM, count per million reads.