Percentages of MVB II, atypical MVB II, and vacuoles labeled with platelet-specific markers in cultured MKs
| Proteins labeled . | Vps33bfl/fl . | Vps33bfl/fl-ERT2 . | ||||
|---|---|---|---|---|---|---|
| MVB II . | Atypical MVB II . | Empty vacuoles . | MVB II . | Atypical MVB II . | Empty vacuoles . | |
| VWF | 35.5 ± 6.5 | 36.8 ± 12.3 | 17.9 ± 9.7 | 17.3 ± 5.2* | 29.5 ± 7.2 | 10.9 ± 4.5 |
| CD63 | 6.8 ± 2.3 | 14.8 ± 6.2 | 27.4 ± 15.3 | 19.5 ± 4.2* | 19.4 ± 5.9 | 27.8 ± 4.7 |
| VWF/CD63 | 35.7 ± 5.5 | 28.9 ± 10.7 | 28.6 ± 13.9 | 19.6 ± 5.3* | 19.1 ± 5.8 | 19.3 ± 10.2 |
| Unlabeled | 22.0 ± 6.1 | 19.5 ± 9.8 | 26.2 ± 15.4 | 43.5 ± 7.6* | 32.0 ± 6.8 | 42.0 ± 7.0 |
| Proteins labeled . | Vps33bfl/fl . | Vps33bfl/fl-ERT2 . | ||||
|---|---|---|---|---|---|---|
| MVB II . | Atypical MVB II . | Empty vacuoles . | MVB II . | Atypical MVB II . | Empty vacuoles . | |
| VWF | 35.5 ± 6.5 | 36.8 ± 12.3 | 17.9 ± 9.7 | 17.3 ± 5.2* | 29.5 ± 7.2 | 10.9 ± 4.5 |
| CD63 | 6.8 ± 2.3 | 14.8 ± 6.2 | 27.4 ± 15.3 | 19.5 ± 4.2* | 19.4 ± 5.9 | 27.8 ± 4.7 |
| VWF/CD63 | 35.7 ± 5.5 | 28.9 ± 10.7 | 28.6 ± 13.9 | 19.6 ± 5.3* | 19.1 ± 5.8 | 19.3 ± 10.2 |
| Unlabeled | 22.0 ± 6.1 | 19.5 ± 9.8 | 26.2 ± 15.4 | 43.5 ± 7.6* | 32.0 ± 6.8 | 42.0 ± 7.0 |
MVB II, atypical MVB II, and vacuoles were counted over cryosections of cultured MKs after immunolabeling with VWF and CD63. The data are expressed as percentages of a structure that contains gold particles over the total number of the selected structure. Fifteen MKs imaged per genotype, 4 to 5 fields of view per MK (4.98 × 3.32 μm). All values are mean ± SEM.
P < .05.