Criteria for lineage assignment for a diagnosis of MPAL
| Lineage assignment criteria . |
|---|
| Myeloid lineage |
| MPO* (flow cytometry, immunohistochemistry, or cytochemistry) |
| or |
| Monocytic differentiation (at least 2 of the following: nonspecific esterase cytochemistry, CD11c, CD14, CD64, lysozyme) |
| T-lineage |
| Strong† cytoplasmic CD3 (with antibodies to CD3 ε chain) |
| or |
| Surface CD3 |
| B-lineage |
| Strong† CD19 with at least 1 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 |
| or |
| Weak CD19 with at least 2 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 |
| Lineage assignment criteria . |
|---|
| Myeloid lineage |
| MPO* (flow cytometry, immunohistochemistry, or cytochemistry) |
| or |
| Monocytic differentiation (at least 2 of the following: nonspecific esterase cytochemistry, CD11c, CD14, CD64, lysozyme) |
| T-lineage |
| Strong† cytoplasmic CD3 (with antibodies to CD3 ε chain) |
| or |
| Surface CD3 |
| B-lineage |
| Strong† CD19 with at least 1 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 |
| or |
| Weak CD19 with at least 2 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 |