Relative expression of GNB isoforms in different shRNA-transduced HL60 cell lines
| . | GNB1 (SD) . | GNB2 (SD) . | GNB4 (SD) . | GNB5 (SD) . |
|---|---|---|---|---|
| shGNB1 | 0.37 (± 0.15)* | 1.13 (± 0.19) | 1.07 (± 0.28) | 1.19 (± 0.06) |
| shGNB2 | 1.46 (± 0.29) | 0.05 (± 0.01)* | 0.89 (± 0.13) | 0.93 (± 0.11) |
| shGNB4 | 1.14 (± 0.08) | 1.29 (± 0.09) | 0.12 (± 0.02)* | 1.27 (± 0.21) |
| shGNB5 | 1.15 (± 0.16) | 1.02 (± 0.17) | 0.95 (± 0.11) | 0.10 (± 0.03)* |
| . | GNB1 (SD) . | GNB2 (SD) . | GNB4 (SD) . | GNB5 (SD) . |
|---|---|---|---|---|
| shGNB1 | 0.37 (± 0.15)* | 1.13 (± 0.19) | 1.07 (± 0.28) | 1.19 (± 0.06) |
| shGNB2 | 1.46 (± 0.29) | 0.05 (± 0.01)* | 0.89 (± 0.13) | 0.93 (± 0.11) |
| shGNB4 | 1.14 (± 0.08) | 1.29 (± 0.09) | 0.12 (± 0.02)* | 1.27 (± 0.21) |
| shGNB5 | 1.15 (± 0.16) | 1.02 (± 0.17) | 0.95 (± 0.11) | 0.10 (± 0.03)* |
Values indicate x-fold induction of the expression of the respective molecules in comparison with cells transduced with a nonsilencing scrambled construct. Expression levels were evaluated in a quantitative reverse-transcription polymerase chain reaction using gene-specific primer and probes (n = 4 experiments).
SD, standard deviation.
P < .05 vs gene expression in scrambled cells.