Relative expression of surface molecules in different shRNA-transduced HL60 cell lines
| . | CD11a (SD) . | CD11b (SD) . | CD62L (SD) . | CD162 (SD) . | CXCR2 (SD) . |
|---|---|---|---|---|---|
| shGNB1 | 1.15 (± 0.04) | 1.32 (± 0.23) | 0.90 (± 0.07) | 1.20 (± 0.08) | 1.13 (± 0.39) |
| shGNB2 | 0.77 (± 0.03) | 1.20 (± 0.03) | 0.82 (± 0.11) | 1.37 (± 0.11) | 0.96 (± 0.23) |
| shGNB4 | 0.89 (± 0.07) | 1.02 (± 0.25) | 0.86 (± 0.22) | 0.94 (± 0.32) | 1.06 (± 0.26) |
| shGNB5 | 0.97 (± 0.29) | 0.81 (± 0.06) | 0.98 (± 0.18) | 0.85 (± 0.21) | 0.95 (± 0.28) |
| shGNAI2 | 0.87 (± 0.32) | 1.10 (± 0.12) | 0.92 (± 0.07) | 0.95 (± 0.19) | 1.10 (± 0.14) |
| . | CD11a (SD) . | CD11b (SD) . | CD62L (SD) . | CD162 (SD) . | CXCR2 (SD) . |
|---|---|---|---|---|---|
| shGNB1 | 1.15 (± 0.04) | 1.32 (± 0.23) | 0.90 (± 0.07) | 1.20 (± 0.08) | 1.13 (± 0.39) |
| shGNB2 | 0.77 (± 0.03) | 1.20 (± 0.03) | 0.82 (± 0.11) | 1.37 (± 0.11) | 0.96 (± 0.23) |
| shGNB4 | 0.89 (± 0.07) | 1.02 (± 0.25) | 0.86 (± 0.22) | 0.94 (± 0.32) | 1.06 (± 0.26) |
| shGNB5 | 0.97 (± 0.29) | 0.81 (± 0.06) | 0.98 (± 0.18) | 0.85 (± 0.21) | 0.95 (± 0.28) |
| shGNAI2 | 0.87 (± 0.32) | 1.10 (± 0.12) | 0.92 (± 0.07) | 0.95 (± 0.19) | 1.10 (± 0.14) |
Values indicate x-fold induction of the expression of the different surface molecules in comparison with cells transduced with a nonsilencing scrambled construct. Expression levels were evaluated by flow cytometry. Differences among different cell lines are not statistically significant (n = 4 experiments).
SD, standard deviation.