Immunophenotypic markers used for the identification and characterization of BM MSCs and other BM cell populations
| Marker . | Fluorochrome . | Clone . | Source* . |
|---|---|---|---|
| CD3 | PB | UCHT1 | BD Biosciences |
| CD10 | APC-H7 | HI10A | BD Biosciences |
| CD11b | FITC | Bear1 | Beckman Coulter |
| CD13 | APC/PE | WM15/L138 | BD Biosciences |
| CD14 | APC-H7 | MφP9 | BD Biosciences |
| CD19 | APC-H7 | SJ25C1 | BD Biosciences |
| CD34 | PerCP- Cy5.5/APC | 8G12 | BD Biosciences |
| CD44 | PerCP-Cy5.5 | IM7 | eBioscience |
| CD45 | PO/OC515 | HI30/GA90 | Invitrogen/Cytognos |
| CD73 | PE | AD2 | BD Biosciences |
| CD90 | PE | 5E10 | BD Biosciences |
| CD105 | FITC/PE | 166707/1G2 | R&D Systems/ Beckman Coulter |
| CD117 | PE Cy7 | 104D2D1 | Beckman Coulter |
| CD140b | PE | 28D4 | BD Biosciences |
| CD146 | PE | P1H12 | BD Biosciences |
| CD271 | PE Cy7 | ME20.4 | Biolegend |
| HLA-DR | PB | L243 | Biolegend |
| MSCA-1 | PE | W8B2 | Miltenyi Biotec |
| SSEA-4 | PE | MC-813-70 | Biolegend |
| STRO-1 | FITC | STRO-1 | Biolegend |
| Marker . | Fluorochrome . | Clone . | Source* . |
|---|---|---|---|
| CD3 | PB | UCHT1 | BD Biosciences |
| CD10 | APC-H7 | HI10A | BD Biosciences |
| CD11b | FITC | Bear1 | Beckman Coulter |
| CD13 | APC/PE | WM15/L138 | BD Biosciences |
| CD14 | APC-H7 | MφP9 | BD Biosciences |
| CD19 | APC-H7 | SJ25C1 | BD Biosciences |
| CD34 | PerCP- Cy5.5/APC | 8G12 | BD Biosciences |
| CD44 | PerCP-Cy5.5 | IM7 | eBioscience |
| CD45 | PO/OC515 | HI30/GA90 | Invitrogen/Cytognos |
| CD73 | PE | AD2 | BD Biosciences |
| CD90 | PE | 5E10 | BD Biosciences |
| CD105 | FITC/PE | 166707/1G2 | R&D Systems/ Beckman Coulter |
| CD117 | PE Cy7 | 104D2D1 | Beckman Coulter |
| CD140b | PE | 28D4 | BD Biosciences |
| CD146 | PE | P1H12 | BD Biosciences |
| CD271 | PE Cy7 | ME20.4 | Biolegend |
| HLA-DR | PB | L243 | Biolegend |
| MSCA-1 | PE | W8B2 | Miltenyi Biotec |
| SSEA-4 | PE | MC-813-70 | Biolegend |
| STRO-1 | FITC | STRO-1 | Biolegend |
APC, allophycocyanin; Cy7, cyanine 7; FITC, fluorescein isothiocyanate; H7, Hilite7; OC515, Orange Cytognos 515; PB, Pacific blue; PO, Pacific orange; PE, phycoerythrin; PE Cy7, phycoerythrin cyanine 7; PerCP Cy5.5, peridinin chlorophyll protein–cyanine 5.5.
Source locations: BD Biosciences (San Diego, CA); Beckman Coulter (Brea, CA); eBioscience (San Diego, CA); Invitrogen (Carlsbad, CA); Cytognos SL (Salamanca, Spain); R&D Systems (Minneapolis, MN); Biolegend (San Diego, CA); Miltenyi Biotec (Bergisch Gladbach, Germany).