Characteristics of HLA class I Tg NSG recipients
| Immunization group recipient ID . | CB ID . | HLA . | PB chimerism, % . | Spl chimerism, % . | Spl CD3+/hCD45+, % . | Spl CD33+/hCD45+, % . | Spl WT1 tet+/CD8+, % . |
|---|---|---|---|---|---|---|---|
| No immunization (n = 10) | |||||||
| A2-1 | 1-030304 | A2+A24+* | 39.6 | 92.2 | 72.4 | 4.2 | 0.039 |
| A2-2 | 23-4 | A0201/0206 | 93.4 | 94.0 | 57.4 | 4.3 | 0.015 |
| A2-3 | 23-35 | A0201/0206 | 60.1 | 84.1 | 89.2 | 2.4 | 0.000 |
| A2-4 | 23-35 | A0201/0206 | 65.7 | 77.1 | 84.4 | 2.0 | 0.029 |
| A24-1 | 22-52 | A2−A24+* | 96.1 | 99.1 | 81.0 | 1.5 | 0.062 |
| A24-2 | 22-58 | A0206/2402 | 76.1 | 98.3 | 69.2 | 2.7 | 0.071 |
| A24-3 | 23-3 | A0206/2402 | 81.2 | 92.4 | 39.3 | 4.6 | 0.061 |
| A24-4 | 23-11 | A1101/2402 | 94.6 | 98.5 | 87.7 | 1.3 | 0.045 |
| A24-5 | 24-13 | A2402/− | 64.8 | 88.8 | 19.2 | 2.5 | 0.048 |
| A24-6 | 24-19 | A2402/3303 | 30.9 | 87.9 | 59.7 | 4.2 | 0.041 |
| Mean ± SE | 70.3 ± 7.1 | 91.2 ± 2.2 | 65.9 ± 7.2 | 3.0 ± 0.4 | 0.041 ± 0.007 | ||
| Immunized without DCs (n = 10) | |||||||
| A2-5 | 20-71 | A0206/2402 | 23.7 | NA | NA | NA | 0.208 |
| A2-6 | 22-7 | A2+A24−* | 78.9 | 95.2 | 58.5 | 5.3 | 0.050 |
| A2-7 | 22-18 | A2+A24+* | 97.7 | 99.2 | 64.4 | 10.6 | 0.030 |
| A24-7 | 22-58 | A0206/2402 | 47.1 | 94.5 | 69.8 | 3.8 | 0.353 |
| A24-8 | 22-31 | A2402/3201 | 75.2 | 75.4 | 79.1 | 1.5 | 0.116 |
| A24-9 | 22-29 | A2−A24+* | 44.1 | 89.8 | 36.5 | 4.7 | 0.022 |
| A24-10 | 23-3 | A0206/2402 | 63.5 | 89.8 | 22.5 | 4.3 | 0.035 |
| A24-11 | 23-3 | A0206/2402 | 50.1 | 74.7 | 14.7 | 5.5 | 0.287 |
| A24-12 | 23-11 | A1101/2402 | 15.1 | 62.7 | 39.3 | 4.5 | 0.252 |
| A24-13 | 24-13 | A2402/− | 58.0 | 92.6 | 13.5 | 1.6 | 0.349 |
| Mean ± SE | 55.3 ± 7.9 | 86.0 ± 4.1 | 44.2 ± 8.2 | 4.7 ± 0.9 | 0.170 ± 0.043 | ||
| Immunized with DCs (n = 9) | |||||||
| A2-8 | 20-28 | A0201/3101 | 55.8 | NA | NA | NA | 0.503 |
| A2-9 | 07-329 | A0202/3303 | 58.9 | NA | NA | NA | 0.072 |
| A2-10 | 22-63 | A2+A24−* | 53.8 | 79.4 | 44.2 | 3.3 | 0.213 |
| A24-14 | 22-52 | A2−A24+* | 66.2 | 92.5 | 72.6 | 4.1 | 0.201 |
| A24-15 | 22-29 | A2−A24+* | 70.2 | 93.5 | 5.9 | 7.0 | 0.334 |
| A24-16 | 23-3 | A0206/2402 | 39.3 | 83.1 | 13.8 | 1.6 | 0.248 |
| A24-17 | 23-3 | A0206/2402 | 84.5 | 87.1 | 78.4 | 3.8 | 0.056 |
| A24-18 | 23-16 | A2402/3303 | 93.6 | 95.6 | 93.0 | 0.9 | 0.218 |
| A24-19 | 24-13 | A2402/− | 81.9 | 94.9 | 11.9 | 1.0 | 0.222 |
| Mean ± SE | 61.2 ± 8.3 | 78.8 ± 10.3 | 41.0 ± 12.0 | 2.8 ± 0.7 | 0.211 ± 0.046 |
| Immunization group recipient ID . | CB ID . | HLA . | PB chimerism, % . | Spl chimerism, % . | Spl CD3+/hCD45+, % . | Spl CD33+/hCD45+, % . | Spl WT1 tet+/CD8+, % . |
|---|---|---|---|---|---|---|---|
| No immunization (n = 10) | |||||||
| A2-1 | 1-030304 | A2+A24+* | 39.6 | 92.2 | 72.4 | 4.2 | 0.039 |
| A2-2 | 23-4 | A0201/0206 | 93.4 | 94.0 | 57.4 | 4.3 | 0.015 |
| A2-3 | 23-35 | A0201/0206 | 60.1 | 84.1 | 89.2 | 2.4 | 0.000 |
| A2-4 | 23-35 | A0201/0206 | 65.7 | 77.1 | 84.4 | 2.0 | 0.029 |
| A24-1 | 22-52 | A2−A24+* | 96.1 | 99.1 | 81.0 | 1.5 | 0.062 |
| A24-2 | 22-58 | A0206/2402 | 76.1 | 98.3 | 69.2 | 2.7 | 0.071 |
| A24-3 | 23-3 | A0206/2402 | 81.2 | 92.4 | 39.3 | 4.6 | 0.061 |
| A24-4 | 23-11 | A1101/2402 | 94.6 | 98.5 | 87.7 | 1.3 | 0.045 |
| A24-5 | 24-13 | A2402/− | 64.8 | 88.8 | 19.2 | 2.5 | 0.048 |
| A24-6 | 24-19 | A2402/3303 | 30.9 | 87.9 | 59.7 | 4.2 | 0.041 |
| Mean ± SE | 70.3 ± 7.1 | 91.2 ± 2.2 | 65.9 ± 7.2 | 3.0 ± 0.4 | 0.041 ± 0.007 | ||
| Immunized without DCs (n = 10) | |||||||
| A2-5 | 20-71 | A0206/2402 | 23.7 | NA | NA | NA | 0.208 |
| A2-6 | 22-7 | A2+A24−* | 78.9 | 95.2 | 58.5 | 5.3 | 0.050 |
| A2-7 | 22-18 | A2+A24+* | 97.7 | 99.2 | 64.4 | 10.6 | 0.030 |
| A24-7 | 22-58 | A0206/2402 | 47.1 | 94.5 | 69.8 | 3.8 | 0.353 |
| A24-8 | 22-31 | A2402/3201 | 75.2 | 75.4 | 79.1 | 1.5 | 0.116 |
| A24-9 | 22-29 | A2−A24+* | 44.1 | 89.8 | 36.5 | 4.7 | 0.022 |
| A24-10 | 23-3 | A0206/2402 | 63.5 | 89.8 | 22.5 | 4.3 | 0.035 |
| A24-11 | 23-3 | A0206/2402 | 50.1 | 74.7 | 14.7 | 5.5 | 0.287 |
| A24-12 | 23-11 | A1101/2402 | 15.1 | 62.7 | 39.3 | 4.5 | 0.252 |
| A24-13 | 24-13 | A2402/− | 58.0 | 92.6 | 13.5 | 1.6 | 0.349 |
| Mean ± SE | 55.3 ± 7.9 | 86.0 ± 4.1 | 44.2 ± 8.2 | 4.7 ± 0.9 | 0.170 ± 0.043 | ||
| Immunized with DCs (n = 9) | |||||||
| A2-8 | 20-28 | A0201/3101 | 55.8 | NA | NA | NA | 0.503 |
| A2-9 | 07-329 | A0202/3303 | 58.9 | NA | NA | NA | 0.072 |
| A2-10 | 22-63 | A2+A24−* | 53.8 | 79.4 | 44.2 | 3.3 | 0.213 |
| A24-14 | 22-52 | A2−A24+* | 66.2 | 92.5 | 72.6 | 4.1 | 0.201 |
| A24-15 | 22-29 | A2−A24+* | 70.2 | 93.5 | 5.9 | 7.0 | 0.334 |
| A24-16 | 23-3 | A0206/2402 | 39.3 | 83.1 | 13.8 | 1.6 | 0.248 |
| A24-17 | 23-3 | A0206/2402 | 84.5 | 87.1 | 78.4 | 3.8 | 0.056 |
| A24-18 | 23-16 | A2402/3303 | 93.6 | 95.6 | 93.0 | 0.9 | 0.218 |
| A24-19 | 24-13 | A2402/− | 81.9 | 94.9 | 11.9 | 1.0 | 0.222 |
| Mean ± SE | 61.2 ± 8.3 | 78.8 ± 10.3 | 41.0 ± 12.0 | 2.8 ± 0.7 | 0.211 ± 0.046 |
NA, not available; SE, standard error.
HLA-A genotypes were not available for these CB mononuclear cells. Their flow cytometric phenotypes of HLA-A2 and HLA-A24 are shown.