NSG-HLA-A24/HHD recipients transplanted with WT1-TCR-transduced HSCs
| Recipient ID . | CB ID . | HLA . | PB chimerism, % . | PB GFP+/hCD45+,% . | Spl chimerism, % . | Spl GFP+/hCD45+, % . | Spl CD3+/hCD45+, % . | Spl WT1 tet+/CD8+, % . |
|---|---|---|---|---|---|---|---|---|
| Nontransduced (n = 4) | ||||||||
| A24-20 | 23-1 | A1101/2601 | 60.0 | — | 84.3 | — | 68.9 | 0.067 |
| A24-21 | 23-44 | A0206/3303 | 87.3 | — | 97.9 | — | 35.3 | 0.021 |
| A24-22 | 23-44 | A0206/3303 | 81.2 | — | 95.3 | — | 92.8 | 0.015 |
| A24-23 | 24-11 | A2402/− | 80.3 | — | 94.3 | — | 26.7 | 0.048 |
| Mean ± SE | 77.2 ± 5.9 | — | 93.0 ± 3.0 | — | 55.9 ± 15.3 | 0.038 ± 0.012 | ||
| GFP control (n = 3) | ||||||||
| A24-GFP-1 | 24-7 | A3101/− | 18.5 | 90.3 | 64.4 | 98.8 | 12.7 | 0.000 |
| A24-GFP-2 | 24-7 | A3101/− | 1.1 | 100.0 | 2.3 | 99.4 | 1.2 | NA |
| A24-GFP-3 | 24-21 | A2402/− | 57.7 | 14.0 | 87.1 | 22.4 | 46.7 | 0.013 |
| Mean ± SE | 25.8 ± 16.7 | 68.1 ± 27.2 | 51.3 ± 25.4 | 73.6 ± 25.6 | 20.2 ± 13.6 | 0.006 ± 0.006 | ||
| WT1-TCR (n = 15) | ||||||||
| A24-TCR-1 | 23-1 | A1101/2601 | 47.0 | 7.5 | 80.9 | 31.6 | 50.2 | 0.455 |
| A24-TCR-2 | 23-1 | A1101/2601 | 63.9 | 25.7 | 90.2 | 33.8 | 78.6 | 0.186 |
| A24-TCR-3 | 23-44 | A0206/3303 | 45.3 | 21.3 | 79.9 | 32.1 | 25.4 | 0.216 |
| A24-TCR-4 | 23-44 | A0206/3303 | 51.5 | 20.2 | 67.4 | 27.8 | 10.9 | 0.165 |
| A24-TCR-5 | 23-44 | A0206/3303 | 53.0 | 24.7 | 86.8 | 36.3 | 18.4 | 0.118 |
| A24-TCR-6 | 23-44 | A0206/3303 | 27.3 | 27.2 | 75.9 | 73.2 | 0.7 | NA |
| A24-TCR-7 | 24-7 | A3101/− | 9.1 | 42.7 | 48.0 | 83.2 | 0.3 | NA |
| A24-TCR-8 | 24-7 | A3101/− | 0.6 | 62.2 | 11.0 | 79.0 | 3.3 | NA |
| A24-TCR-9 | 24-13 | A2402/− | 78.5 | 62.8 | 89.4 | 74.5 | 9.9 | 0.636 |
| A24-TCR-10 | 24-15 | A3101/3303 | 50.7 | 94.6 | 76.4 | 92.8 | 1.4 | 0.585 |
| A24-TCR-11 | 24-21 | A2402/− | 51.5 | 2.0 | 87.2 | 7.2 | 68.4 | 0.073 |
| A24-TCR-12 | 24-16 | A0201/3303 | 11.9 | 92.1 | 34.0 | 95.8 | 13.5 | NA |
| A24-TCR-13 | 24-16 | A0201/3303 | 28.3 | 18.1 | 74.2 | 64.5 | 46.7 | 0.410 |
| A24-TCR-14 | 24-15 | A3101/3303 | 10.1 | 99.0 | 54.6 | 98.7 | 2.3 | NA |
| A24-TCR-15 | 24-40 | A2402/− | 16.2 | 90.2 | 58.6 | 95.7 | 1.0 | NA |
| Mean ± SE | 36.3 ± 6.2 | 46.0 ± 9.2 | 67.6 ± 6.1 | 61.8 ± 8.2 | 22.1 ± 7.1 | 0.316 ± 0.070 |
| Recipient ID . | CB ID . | HLA . | PB chimerism, % . | PB GFP+/hCD45+,% . | Spl chimerism, % . | Spl GFP+/hCD45+, % . | Spl CD3+/hCD45+, % . | Spl WT1 tet+/CD8+, % . |
|---|---|---|---|---|---|---|---|---|
| Nontransduced (n = 4) | ||||||||
| A24-20 | 23-1 | A1101/2601 | 60.0 | — | 84.3 | — | 68.9 | 0.067 |
| A24-21 | 23-44 | A0206/3303 | 87.3 | — | 97.9 | — | 35.3 | 0.021 |
| A24-22 | 23-44 | A0206/3303 | 81.2 | — | 95.3 | — | 92.8 | 0.015 |
| A24-23 | 24-11 | A2402/− | 80.3 | — | 94.3 | — | 26.7 | 0.048 |
| Mean ± SE | 77.2 ± 5.9 | — | 93.0 ± 3.0 | — | 55.9 ± 15.3 | 0.038 ± 0.012 | ||
| GFP control (n = 3) | ||||||||
| A24-GFP-1 | 24-7 | A3101/− | 18.5 | 90.3 | 64.4 | 98.8 | 12.7 | 0.000 |
| A24-GFP-2 | 24-7 | A3101/− | 1.1 | 100.0 | 2.3 | 99.4 | 1.2 | NA |
| A24-GFP-3 | 24-21 | A2402/− | 57.7 | 14.0 | 87.1 | 22.4 | 46.7 | 0.013 |
| Mean ± SE | 25.8 ± 16.7 | 68.1 ± 27.2 | 51.3 ± 25.4 | 73.6 ± 25.6 | 20.2 ± 13.6 | 0.006 ± 0.006 | ||
| WT1-TCR (n = 15) | ||||||||
| A24-TCR-1 | 23-1 | A1101/2601 | 47.0 | 7.5 | 80.9 | 31.6 | 50.2 | 0.455 |
| A24-TCR-2 | 23-1 | A1101/2601 | 63.9 | 25.7 | 90.2 | 33.8 | 78.6 | 0.186 |
| A24-TCR-3 | 23-44 | A0206/3303 | 45.3 | 21.3 | 79.9 | 32.1 | 25.4 | 0.216 |
| A24-TCR-4 | 23-44 | A0206/3303 | 51.5 | 20.2 | 67.4 | 27.8 | 10.9 | 0.165 |
| A24-TCR-5 | 23-44 | A0206/3303 | 53.0 | 24.7 | 86.8 | 36.3 | 18.4 | 0.118 |
| A24-TCR-6 | 23-44 | A0206/3303 | 27.3 | 27.2 | 75.9 | 73.2 | 0.7 | NA |
| A24-TCR-7 | 24-7 | A3101/− | 9.1 | 42.7 | 48.0 | 83.2 | 0.3 | NA |
| A24-TCR-8 | 24-7 | A3101/− | 0.6 | 62.2 | 11.0 | 79.0 | 3.3 | NA |
| A24-TCR-9 | 24-13 | A2402/− | 78.5 | 62.8 | 89.4 | 74.5 | 9.9 | 0.636 |
| A24-TCR-10 | 24-15 | A3101/3303 | 50.7 | 94.6 | 76.4 | 92.8 | 1.4 | 0.585 |
| A24-TCR-11 | 24-21 | A2402/− | 51.5 | 2.0 | 87.2 | 7.2 | 68.4 | 0.073 |
| A24-TCR-12 | 24-16 | A0201/3303 | 11.9 | 92.1 | 34.0 | 95.8 | 13.5 | NA |
| A24-TCR-13 | 24-16 | A0201/3303 | 28.3 | 18.1 | 74.2 | 64.5 | 46.7 | 0.410 |
| A24-TCR-14 | 24-15 | A3101/3303 | 10.1 | 99.0 | 54.6 | 98.7 | 2.3 | NA |
| A24-TCR-15 | 24-40 | A2402/− | 16.2 | 90.2 | 58.6 | 95.7 | 1.0 | NA |
| Mean ± SE | 36.3 ± 6.2 | 46.0 ± 9.2 | 67.6 ± 6.1 | 61.8 ± 8.2 | 22.1 ± 7.1 | 0.316 ± 0.070 |
Abbreviations are explained in Table 1.
–, no GFP+ cells in the nontransduced group.