LSC frequency analysis for paired diagnosis and relapse samples.
| AML . | Status . | Cells injected, n . | Frequency . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1E3 . | 5E3 . | 1E4 . | 5E4 . | 1E5 . | 5E5 . | 1E6 . | 5E6 . | |||
| AML1 | Diagnosis | — | — | — | 0/4 | 0/3 | 3/4 | 3/3 | 1/1 | 428 455 |
| AML1 | Relapse | — | — | 3/3 | 5/5 | 5/5 | 5/5 | 5/5 | 4/4 | <10 000 |
| AML3 | Diagnosis | — | 3/5 | 2/4 | 5/5 | 5/5 | 5/5 | 3/3 | — | 8 815 |
| AML3 | Relapse | 4/4 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 2/2 | — | <1 000 |
| AML15 | Diagnosis | — | — | 0/5 | 0/5 | 1/5 | 1/5 | 1/2 | 4/5 | 2 297 888 |
| AML15 | Relapse | 0/5 | 1/5 | 1/5 | 1/4 | 3/5 | 5/5 | 2/2 | — | 92 862 |
| AML18 | Diagnosis | — | 1/5 | 1/4 | 3/5 | 2/3 | 4/4 | 1/1 | — | 56 116 |
| AML18 | Relapse | 4/5 | 5/5 | 3/3 | 4/4 | 4/4 | — | — | — | 619 |
| AML22 | Diagnosis | — | 0/2 | 1/3 | 1/5 | 2/4 | 2/4 | 1/2 | 1/1 | 472 106 |
| AML22 | Relapse | 0/3 | 1/3 | 4/5 | 2/2 | 2/4 | 3/3 | 2/2 | — | 38 300 |
| AML . | Status . | Cells injected, n . | Frequency . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1E3 . | 5E3 . | 1E4 . | 5E4 . | 1E5 . | 5E5 . | 1E6 . | 5E6 . | |||
| AML1 | Diagnosis | — | — | — | 0/4 | 0/3 | 3/4 | 3/3 | 1/1 | 428 455 |
| AML1 | Relapse | — | — | 3/3 | 5/5 | 5/5 | 5/5 | 5/5 | 4/4 | <10 000 |
| AML3 | Diagnosis | — | 3/5 | 2/4 | 5/5 | 5/5 | 5/5 | 3/3 | — | 8 815 |
| AML3 | Relapse | 4/4 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 2/2 | — | <1 000 |
| AML15 | Diagnosis | — | — | 0/5 | 0/5 | 1/5 | 1/5 | 1/2 | 4/5 | 2 297 888 |
| AML15 | Relapse | 0/5 | 1/5 | 1/5 | 1/4 | 3/5 | 5/5 | 2/2 | — | 92 862 |
| AML18 | Diagnosis | — | 1/5 | 1/4 | 3/5 | 2/3 | 4/4 | 1/1 | — | 56 116 |
| AML18 | Relapse | 4/5 | 5/5 | 3/3 | 4/4 | 4/4 | — | — | — | 619 |
| AML22 | Diagnosis | — | 0/2 | 1/3 | 1/5 | 2/4 | 2/4 | 1/2 | 1/1 | 472 106 |
| AML22 | Relapse | 0/3 | 1/3 | 4/5 | 2/2 | 2/4 | 3/3 | 2/2 | — | 38 300 |
LDA was performed by injecting increasing numbers of unsorted unfractionated leukemic marrow cells from 5 paired primary patient samples at diagnosis and in relapse into recipient mice (range, 1000-5 000 000). The number of mice demonstrating the presence of human leukemia in their marrow at time of harvest (numerator), as well as the number of mice transplanted (denominator), is indicated for each cell dose for each sample. LSC frequency, calculated using L-Calc Software (STEMCELL Technologies), is reported, as well as the upper and lower estimates for each sample.
1E3, 1000; 5E3, 5000; 1E4, 10 000, 5E4, 50 000; 1E5, 100 000; 5E5, 500 000; 1E6, 1 000 000; 5E6, 5 000 000.