Oligonucleotide primers used for engineering the anti-FcμR-scFv-Fc-CD28-CD3ζ and anti-FcμR-scFv-Fc
| Number . | Orientation . | Name . | 3′-5′ Sequence . |
|---|---|---|---|
| 765 | Antisense | rIgG2ab-1 | AATTTTCTTGTCCACCTTGG |
| 884 | Antisense | CL1FOR | ACACTCAGCACGGGACAAACTCTTCTC |
| 885 | Antisense | CL2FOR | ACACTCTGCAGGAGACAGACTCTTTTC |
| 766 | Antisense | HBS-rG2a | CGTCATGTCGACGGATCCAAGCTTAATAGCCCTTGACCAGGCAT |
| 768 | Sense | rIgG-anc | CGTCGATGAGCTCTAGAATTCGCATGTGCAAGTCCGATG |
| GTCCCCCCCCCCCCCC | |||
| 877 | Antisense | rIgCl-1 | TGTCACCATACACACCAGTGTAGCC |
| 878 | Antisense | rIgCl-2 | ATCAGAAATCAGACACACCAGTGTGGCT |
| 777 | Antisense | rev_pcR2.1_rVh | TGAGGAGACTGTGACCATGACTCCT |
| 778 | Sense | for_pcR2.1_rVh | AGACTGCCATGGATTTTCAGGTGCAGATTTTCAGCTTCCTGCTAAT |
| CAGTGCCTCAGTCATAATGTCTAGAGAGGTGCAGCTCGTGGAAACAG | |||
| 892 | Sense | FcμRla-for | CGGAGTCATGGTCACAGTCTCCTCAGGAGGTGGTGGATCGGGCGGT |
| GGCGGGTCGGGTGGCGGCGGATCTAGCTATGAGCCTGATCCAACCACCTTC | |||
| 893 | Antisense | FcμRla-rev | CTTATCGGATCCCCTAGGACAGTGAGCTTGGTTCC |
| 782 | Sense | for-flank-assVh | AGACTGCCATGGATTTTCAGGTGCAGATTTTCAGCTTCCTGCTAA |
| Number . | Orientation . | Name . | 3′-5′ Sequence . |
|---|---|---|---|
| 765 | Antisense | rIgG2ab-1 | AATTTTCTTGTCCACCTTGG |
| 884 | Antisense | CL1FOR | ACACTCAGCACGGGACAAACTCTTCTC |
| 885 | Antisense | CL2FOR | ACACTCTGCAGGAGACAGACTCTTTTC |
| 766 | Antisense | HBS-rG2a | CGTCATGTCGACGGATCCAAGCTTAATAGCCCTTGACCAGGCAT |
| 768 | Sense | rIgG-anc | CGTCGATGAGCTCTAGAATTCGCATGTGCAAGTCCGATG |
| GTCCCCCCCCCCCCCC | |||
| 877 | Antisense | rIgCl-1 | TGTCACCATACACACCAGTGTAGCC |
| 878 | Antisense | rIgCl-2 | ATCAGAAATCAGACACACCAGTGTGGCT |
| 777 | Antisense | rev_pcR2.1_rVh | TGAGGAGACTGTGACCATGACTCCT |
| 778 | Sense | for_pcR2.1_rVh | AGACTGCCATGGATTTTCAGGTGCAGATTTTCAGCTTCCTGCTAAT |
| CAGTGCCTCAGTCATAATGTCTAGAGAGGTGCAGCTCGTGGAAACAG | |||
| 892 | Sense | FcμRla-for | CGGAGTCATGGTCACAGTCTCCTCAGGAGGTGGTGGATCGGGCGGT |
| GGCGGGTCGGGTGGCGGCGGATCTAGCTATGAGCCTGATCCAACCACCTTC | |||
| 893 | Antisense | FcμRla-rev | CTTATCGGATCCCCTAGGACAGTGAGCTTGGTTCC |
| 782 | Sense | for-flank-assVh | AGACTGCCATGGATTTTCAGGTGCAGATTTTCAGCTTCCTGCTAA |