Table 1

Primers and conditions for real-time PCR

GenePrimer sequence (5′-3′)Annealing temperature (°C)Product length (bp)
ForwardReverse
Human FcγRIIa ATCATTGTGGCTGTGGTCATTG TGTTTCATAGTCATTGTTGGTTTCTTC 65 160 
Human FcγRIIb ATTCCTGGCTCCTGTTGCTG GAATGGAGTCGCTCTCAGGG 65 144 
Human GAPDH GCACCGTCAAGGCTGAGAAC TGGTGAAGACGCCAGTGGA 65 138 
Mouse FcγRIIb CAGAATGCACACTCTGGAAGC GGGTCCCTTCGCACATCAG 60 169 
Mouse FcγRIII AGGGCCTCCATCTGGACTG GTGGTTCTGGTAATCATGCTCTG 60 160 
Mouse β-actin CGGTTCCGATGCCCTGAGGCTCTT CGTCACACTTCATGATGGAATTGA 60 100 
GenePrimer sequence (5′-3′)Annealing temperature (°C)Product length (bp)
ForwardReverse
Human FcγRIIa ATCATTGTGGCTGTGGTCATTG TGTTTCATAGTCATTGTTGGTTTCTTC 65 160 
Human FcγRIIb ATTCCTGGCTCCTGTTGCTG GAATGGAGTCGCTCTCAGGG 65 144 
Human GAPDH GCACCGTCAAGGCTGAGAAC TGGTGAAGACGCCAGTGGA 65 138 
Mouse FcγRIIb CAGAATGCACACTCTGGAAGC GGGTCCCTTCGCACATCAG 60 169 
Mouse FcγRIII AGGGCCTCCATCTGGACTG GTGGTTCTGGTAATCATGCTCTG 60 160 
Mouse β-actin CGGTTCCGATGCCCTGAGGCTCTT CGTCACACTTCATGATGGAATTGA 60 100 
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