Differential expression of genes involved in immune responses
| Genes involved in immune responses or differentiation . | CD37− vs CD37+ in GCB/ABC DLBCL . | p50high* vs p50low in CD37− GCB/ABC DLBCL . | CXCR4high* vs CXCR4low in CD37+ GCB/ABC DLBCL . | ABC vs GCB in CD37+ DLBCL . |
|---|---|---|---|---|
| ICOSLG | ↓ in GCB (P = .0036) | ↓ (P < .0001) | ||
| CD58 | ↓ in GCB (P = .022) | ↓ in CD37+ ABC (P = .056) | ↓ (P = .0009) | |
| FCGR2A (CD32A) | ↑ in both GCB (P = .016) and ABC (P = .044) | ↑ in both CD37− GCB (P = .0078) and CD37− ABC (P = .016) | ↓ in CD37+ ABC (P = .02) | ↑ (P = .0022) |
| FCGR1B (CD64) | ↑ in both GCB (P = .01) and ABC (P = .0007) | ↑ in both CD37− GCB (P = .0003) and CD37− ABC (P = .0094) | ↑ (P = .0005) | |
| C1QB | ↑ in both GCB (P = .0001) and ABC (P = .0027) | ↑ in both CD37− GCB (P < .0001) and CD37− ABC (P = .0007) | ↑ (P < .0001) | |
| CD8A | ↑ in ABC (P = .0014) | ↑ in both CD37− GCB (P = .017) and CD37− ABC (P = .039) | ||
| CD8B | ↑ in both GCB (P = .068) and in ABC (P = .0012) | ↑ in both CD37− GCB (P = .0059) and CD37− ABC (P = .038) | Trends of ↓ in CD37+ GCB (P = .08) and CD37+ ABC (P = .068) | |
| PDCD1 (PD-1) | ↑ in ABC (P = .03); ↑ in p50high GCB (P = .05) | ↑ in CD37− ABC (P = .03) | ↓ in CD37+ GCB (P = .014) and trend of ↓ in CD37+ ABC (P = .069) | |
| CD274 (PD-L1) | ↑ in CD37− GCB (P = .013) | ↑ in CD37+ ABC (P = .018) | ||
| PDCD1LG2 (PD-L2) | Trend of ↑ in ABC (P = .09) | ↑ in CD37− GCB (P = .0025) | ||
| CTLA4 | ↑ in both CD37− GCB (P = .027) and CD37− ABC (P = .0032) | ↓ in CD37+ ABC (P = .0005); trend of ↓ in CD37+ GCB (P = .086) | ||
| HAVCR2 (TIM3) | ↑ in both CD37− GCB (P = .0027) and CD37− ABC (P = .0001) | |||
| CIITA | ↓ in GCB (P = .014) | ↓ (P < .0001) | ||
| HLA-A-G | (↑ in overall CD37− ABC vs CD37+ ABC, but it is due to the association with p50 and CXCR4) | ↑ in both CD37− GCB (P = .0056) and CD37− ABC (P = .0003) | ↓ in both CD37+ GCB (P = .0073) and CD37+ ABC (P = .025) | |
| LILRB2 (LIR2) | ↑ in both GCB (P = .0014) and ABC (P < .0001) | ↑ in both CD37− GCB (P = .0001) and CD37− ABC (P = .0065) | ↓ in CD37+ GCB (P = .0017) | ↑ (P < .0001) |
| LILRA3 (LIR4) | ↑ in ABC (P = .0021) | ↑ in CD37− GCB (P = .001) | ↑ (P = .0028) | |
| LILRA1 (LIR6) | ↑ in GCB (P = .028) | ↑ in CD37− GCB (P = .0062) | ↑ (P = .0002) | |
| CASP1 | ↑ in ABC (P = .018) | ↑ in both CD37− GCB (P = .041) and CD37− ABC (P = .029) | ↓ in CD37+ ABC (P = .0056) | ↑ (P = .023) |
| CASP7 | ↑ in GCB (P = .02) | ↑ in CD37− ABC (P = .031) | ↑ (P < .0001) | |
| CASP8 | ↑ in GCB (P = .023) | ↑ (P < .0001) | ||
| CASP10 | ↑ in ABC (P = .018); trends of ↑ in GCB (P = .068) | ↓ in CD37+ GCB (P = .0097) | ||
| CXCL12 | ↑ in ABC (P = .0072) | ↑ in both CD37− GCB (P = .025) and CD37− ABC (P = .0007) | ↓ in CD37+ ABC (P = .0002) | |
| IL6R | ↑ in both CD37− GCB (P = .009) and CD37− ABC (P = .0001) | ↓ in CD37+ ABC (P = .049) | ||
| IL10RB | ↑ in ABC (P = .038) | ↑ in CD37− GCB (P = .0005) | ↑ in CD37+ ABC (P = .034) | |
| IL21R | ↓ in GCB (P = .0036) | |||
| IRF1 | ↑ in both GCB (P = .025) and ABC (P = .0005) | ↑ in CD37− ABC (P = .0004) | ↑ (P = .044) | |
| STAT1 | ↑ in ABC (P = .017) | ↑ in both CD37− GCB (P = .0068) and CD37− ABC (P = .0006) | ||
| IFIT3 | ↑ in both GCB (P = .0071) and ABC (P = .0011) | ↑ in CD37− GCB (P < .0001) | ↓ in CD37+ GCB (P = .047) | ↑ (P = .011) |
| CARD16 | ↑ in ABC (P = .0022) | ↓ in CD37+ ABC (P = .022) | ||
| TCL1A | ↓ in ABC (P = .0071) | Trend of ↓ in CD37− ABC (P = .076) | ↑ in CD37+ GCB (P = .036) | |
| AICDA | ↓ in ABC (P < .0001) | ↑ in both CD37+ GCB (P = .028) and CD37+ ABC (P < .0001) | ↑ (P < .0001) | |
| FOXP1 | ↓ in ABC (P < .0001) | ↑ in CD37+ GCB (P = .03) | ↑ (P < .0001) | |
| BCL11A | ↓ in both GCB (P = .0017) and ABC (P < .0001) | ↓ in both CD37− GCB (P = .0058) and CD37− ABC (P = .017) | ↑ in both CD37+ GCB (P = .04) and CD37+ ABC (P = .0006) | |
| LMO2 | ↓ in GCB (P = .0007) | ↓ (P < .0001) | ||
| POU2F2 (OTF2) | ↓ in ABC (P = .0001) | ↑ (P < .0001) |
| Genes involved in immune responses or differentiation . | CD37− vs CD37+ in GCB/ABC DLBCL . | p50high* vs p50low in CD37− GCB/ABC DLBCL . | CXCR4high* vs CXCR4low in CD37+ GCB/ABC DLBCL . | ABC vs GCB in CD37+ DLBCL . |
|---|---|---|---|---|
| ICOSLG | ↓ in GCB (P = .0036) | ↓ (P < .0001) | ||
| CD58 | ↓ in GCB (P = .022) | ↓ in CD37+ ABC (P = .056) | ↓ (P = .0009) | |
| FCGR2A (CD32A) | ↑ in both GCB (P = .016) and ABC (P = .044) | ↑ in both CD37− GCB (P = .0078) and CD37− ABC (P = .016) | ↓ in CD37+ ABC (P = .02) | ↑ (P = .0022) |
| FCGR1B (CD64) | ↑ in both GCB (P = .01) and ABC (P = .0007) | ↑ in both CD37− GCB (P = .0003) and CD37− ABC (P = .0094) | ↑ (P = .0005) | |
| C1QB | ↑ in both GCB (P = .0001) and ABC (P = .0027) | ↑ in both CD37− GCB (P < .0001) and CD37− ABC (P = .0007) | ↑ (P < .0001) | |
| CD8A | ↑ in ABC (P = .0014) | ↑ in both CD37− GCB (P = .017) and CD37− ABC (P = .039) | ||
| CD8B | ↑ in both GCB (P = .068) and in ABC (P = .0012) | ↑ in both CD37− GCB (P = .0059) and CD37− ABC (P = .038) | Trends of ↓ in CD37+ GCB (P = .08) and CD37+ ABC (P = .068) | |
| PDCD1 (PD-1) | ↑ in ABC (P = .03); ↑ in p50high GCB (P = .05) | ↑ in CD37− ABC (P = .03) | ↓ in CD37+ GCB (P = .014) and trend of ↓ in CD37+ ABC (P = .069) | |
| CD274 (PD-L1) | ↑ in CD37− GCB (P = .013) | ↑ in CD37+ ABC (P = .018) | ||
| PDCD1LG2 (PD-L2) | Trend of ↑ in ABC (P = .09) | ↑ in CD37− GCB (P = .0025) | ||
| CTLA4 | ↑ in both CD37− GCB (P = .027) and CD37− ABC (P = .0032) | ↓ in CD37+ ABC (P = .0005); trend of ↓ in CD37+ GCB (P = .086) | ||
| HAVCR2 (TIM3) | ↑ in both CD37− GCB (P = .0027) and CD37− ABC (P = .0001) | |||
| CIITA | ↓ in GCB (P = .014) | ↓ (P < .0001) | ||
| HLA-A-G | (↑ in overall CD37− ABC vs CD37+ ABC, but it is due to the association with p50 and CXCR4) | ↑ in both CD37− GCB (P = .0056) and CD37− ABC (P = .0003) | ↓ in both CD37+ GCB (P = .0073) and CD37+ ABC (P = .025) | |
| LILRB2 (LIR2) | ↑ in both GCB (P = .0014) and ABC (P < .0001) | ↑ in both CD37− GCB (P = .0001) and CD37− ABC (P = .0065) | ↓ in CD37+ GCB (P = .0017) | ↑ (P < .0001) |
| LILRA3 (LIR4) | ↑ in ABC (P = .0021) | ↑ in CD37− GCB (P = .001) | ↑ (P = .0028) | |
| LILRA1 (LIR6) | ↑ in GCB (P = .028) | ↑ in CD37− GCB (P = .0062) | ↑ (P = .0002) | |
| CASP1 | ↑ in ABC (P = .018) | ↑ in both CD37− GCB (P = .041) and CD37− ABC (P = .029) | ↓ in CD37+ ABC (P = .0056) | ↑ (P = .023) |
| CASP7 | ↑ in GCB (P = .02) | ↑ in CD37− ABC (P = .031) | ↑ (P < .0001) | |
| CASP8 | ↑ in GCB (P = .023) | ↑ (P < .0001) | ||
| CASP10 | ↑ in ABC (P = .018); trends of ↑ in GCB (P = .068) | ↓ in CD37+ GCB (P = .0097) | ||
| CXCL12 | ↑ in ABC (P = .0072) | ↑ in both CD37− GCB (P = .025) and CD37− ABC (P = .0007) | ↓ in CD37+ ABC (P = .0002) | |
| IL6R | ↑ in both CD37− GCB (P = .009) and CD37− ABC (P = .0001) | ↓ in CD37+ ABC (P = .049) | ||
| IL10RB | ↑ in ABC (P = .038) | ↑ in CD37− GCB (P = .0005) | ↑ in CD37+ ABC (P = .034) | |
| IL21R | ↓ in GCB (P = .0036) | |||
| IRF1 | ↑ in both GCB (P = .025) and ABC (P = .0005) | ↑ in CD37− ABC (P = .0004) | ↑ (P = .044) | |
| STAT1 | ↑ in ABC (P = .017) | ↑ in both CD37− GCB (P = .0068) and CD37− ABC (P = .0006) | ||
| IFIT3 | ↑ in both GCB (P = .0071) and ABC (P = .0011) | ↑ in CD37− GCB (P < .0001) | ↓ in CD37+ GCB (P = .047) | ↑ (P = .011) |
| CARD16 | ↑ in ABC (P = .0022) | ↓ in CD37+ ABC (P = .022) | ||
| TCL1A | ↓ in ABC (P = .0071) | Trend of ↓ in CD37− ABC (P = .076) | ↑ in CD37+ GCB (P = .036) | |
| AICDA | ↓ in ABC (P < .0001) | ↑ in both CD37+ GCB (P = .028) and CD37+ ABC (P < .0001) | ↑ (P < .0001) | |
| FOXP1 | ↓ in ABC (P < .0001) | ↑ in CD37+ GCB (P = .03) | ↑ (P < .0001) | |
| BCL11A | ↓ in both GCB (P = .0017) and ABC (P < .0001) | ↓ in both CD37− GCB (P = .0058) and CD37− ABC (P = .017) | ↑ in both CD37+ GCB (P = .04) and CD37+ ABC (P = .0006) | |
| LMO2 | ↓ in GCB (P = .0007) | ↓ (P < .0001) | ||
| POU2F2 (OTF2) | ↓ in ABC (P = .0001) | ↑ (P < .0001) |
Differential expression of genes involved in immune responses or differentiation between CD37− and CD37+ patients, between p50high/CD37− and p50low/CD37− patients (CD37− was associated with p50high), between CXCR4high/CD37+ and CXCR4low/CD37+ patients (CD37+ was associated with CXCR4high in ABC-DLBCL), and between CD37+ ABC-DLBCL and CD37+ GCB-DLBCL (CD37+ was associated with ABC cell of origin). The cutoffs for p50high and CXCR4high were ≥20%. The P values were obtained by the unpaired Student t test (2-tailed).