Relative timing of wound pathology and healing events following hemarthrosis in mice with normal or impaired hemostatic potential: time course of onset, peak, and resolution of findings
| . | WT + NS (no. days after hemarthrosis) . | WT + blood (no. days after hemarthrosis) . | Hemophilia B (no. days after hemarthrosis) . |
|---|---|---|---|
| Frank blood | |||
| Onset | Immediate | Immediate | Immediate |
| Peak | Immediate | Immediate | 1-3 |
| Resolved | 1 | 1 | 10 |
| Synovial hyperplasia | |||
| Onset | None | None | 3 |
| Peak | 5* | 5* | 7-14 |
| Resolved | N/A† | N/A† | 56 |
| Angiogenesis (vessel count) | |||
| Onset | None | None | 5 |
| Peak | 7 (minimal)* | 5 (minimal)* | 10 |
| Resolved | N/A† | N/A† | 56 |
| Chondrocyte apoptosis | |||
| Onset | None | None | 3 |
| Peak | None | None | 7 |
| Resolved | None | None | 56 |
| Chondrocyte loss | |||
| Onset | None | None | 7 |
| Peak | None | None | 14 |
| Resolved | None | None | Unresolved |
| Heme iron | |||
| Onset | None | None | 7 |
| Peak | None | None | 28 |
| Resolved | None | None | Unresolved |
| Macrophage | |||
| Onset | 3 | 1 | 3 |
| Peak | 3 | 3 | 7 |
| Resolved | 10 | 10 | 56 |
| . | WT + NS (no. days after hemarthrosis) . | WT + blood (no. days after hemarthrosis) . | Hemophilia B (no. days after hemarthrosis) . |
|---|---|---|---|
| Frank blood | |||
| Onset | Immediate | Immediate | Immediate |
| Peak | Immediate | Immediate | 1-3 |
| Resolved | 1 | 1 | 10 |
| Synovial hyperplasia | |||
| Onset | None | None | 3 |
| Peak | 5* | 5* | 7-14 |
| Resolved | N/A† | N/A† | 56 |
| Angiogenesis (vessel count) | |||
| Onset | None | None | 5 |
| Peak | 7 (minimal)* | 5 (minimal)* | 10 |
| Resolved | N/A† | N/A† | 56 |
| Chondrocyte apoptosis | |||
| Onset | None | None | 3 |
| Peak | None | None | 7 |
| Resolved | None | None | 56 |
| Chondrocyte loss | |||
| Onset | None | None | 7 |
| Peak | None | None | 14 |
| Resolved | None | None | Unresolved |
| Heme iron | |||
| Onset | None | None | 7 |
| Peak | None | None | 28 |
| Resolved | None | None | Unresolved |
| Macrophage | |||
| Onset | 3 | 1 | 3 |
| Peak | 3 | 3 | 7 |
| Resolved | 10 | 10 | 56 |
Onset is defined as the first recorded point at which the value is statistically different from baseline (day 0). Peak is the time point with the highest value. Resolution is the first recorded time point at which there is no longer a statistically significant difference from baseline (day 0).
A trend toward peak score is demonstrated at these time points; however, it is not statistically significantly different from the baseline.
N/A indicates resolution is not applicable for these groups of mice because mean synovial hyperplasia and neoangiogenesis scores were not statistically significantly different from baseline at any time point.