Sequences of qPCR and genotyping oligos
| Gene . | Forward primer . | Reverse primer . |
|---|---|---|
| qPCR primers | ||
| mAlas2 | GCA GCT ATG TTG CTA CGG TC | GATGGG GCA GCG TCC AAT AC |
| mAlad | GTT CTG CAC AGC GGC TAC TT | AGC TGG TTT ACG CCA TAC CTG |
| mßh1-globin | ATC ATG GGA AAC CCC CGG A | GGG TGA ATT CCT TGG CAA AAT GAG T |
| mßmajor-globin | TCT GCT ATC ATG GGT AAT GCC AAA | GAA GGC AGC CTG TGC AGC G |
| mCdkn1c | AGAGAACTGCGCAGGAGAAC | TCTGGCCGTTAGCCTCTAAA |
| mεY-globin | ACC CTC ATC AAT GGC CTG TGG A | TTC ATT GCC GAA GTG ACT AGC C |
| mTr2 | GAT TCT TTA AAA GAA GCA TCC G | ATG TAG ATT TTT TCT GTT GAA GC |
| mTr4 | TCA GTT GTG AAG GTT GCA AAG G | ACA ATT GCT TGG TTT CTC CCG |
| Genotyping primers | ||
| Wt Tr4 | AGG CAG ACG TCC AGC GG | GTT GCT CCT TGA TAC GTG CCA |
| Null Tr4 | GAC ATC TCT TCA GAC TCT ATA TCT GTT TAA AA | GTT GCT CCT TGA TAC GTG CCA |
| Gene . | Forward primer . | Reverse primer . |
|---|---|---|
| qPCR primers | ||
| mAlas2 | GCA GCT ATG TTG CTA CGG TC | GATGGG GCA GCG TCC AAT AC |
| mAlad | GTT CTG CAC AGC GGC TAC TT | AGC TGG TTT ACG CCA TAC CTG |
| mßh1-globin | ATC ATG GGA AAC CCC CGG A | GGG TGA ATT CCT TGG CAA AAT GAG T |
| mßmajor-globin | TCT GCT ATC ATG GGT AAT GCC AAA | GAA GGC AGC CTG TGC AGC G |
| mCdkn1c | AGAGAACTGCGCAGGAGAAC | TCTGGCCGTTAGCCTCTAAA |
| mεY-globin | ACC CTC ATC AAT GGC CTG TGG A | TTC ATT GCC GAA GTG ACT AGC C |
| mTr2 | GAT TCT TTA AAA GAA GCA TCC G | ATG TAG ATT TTT TCT GTT GAA GC |
| mTr4 | TCA GTT GTG AAG GTT GCA AAG G | ACA ATT GCT TGG TTT CTC CCG |
| Genotyping primers | ||
| Wt Tr4 | AGG CAG ACG TCC AGC GG | GTT GCT CCT TGA TAC GTG CCA |
| Null Tr4 | GAC ATC TCT TCA GAC TCT ATA TCT GTT TAA AA | GTT GCT CCT TGA TAC GTG CCA |
The gene name and sequences for the forward and reverse oligo sequences are listed for each primer pair used in these experiments for qPCR and genotyping.
qPCR, quantitative polymerase chain reaction.