Table 2. Sphingolipids in RBCs, plasma,... | American Society of Hematology

Table 2.

Sphingolipids in RBCs, plasma, and livers of HNF1A^+/+, HNF1A^+/−, and HNF1A^−/− mice

Sphingolipids and metabolites	HNF1A^+/+	HNF1A^+/−	HNF1A^−/−
Erythrocytes, nM	n = 13	n = 4	n = 7
Sphingosine	5 107 ± 729	7 325 ± 324	9 691 ± 1 328**
C16-ceramide	364 ± 33	438 ± 39	633 ± 39***
Lysophosphatidylcholine	68 985 ± 11 401	65 250 ± 8 128	237 914 ± 86 273*
Sphingomyelin	112 215 ± 14 145	115 150 ± 22 291	67 200 ± 8 990*
Phosphatidylcholine	162 477 ± 20 081	179 450 ± 31 295	139 857 ± 34 142
Plasma, nM	n = 6	n = 6	n = 5
Sphingosine	9 ± 1	11 ± 2	25 ± 1***
Sphingosine-1-phosphate	1 233 ± 56	1 027 ± 55†	772 ± 61***
C16-ceramide	31 ± 5	34 ± 3	35 ± 8
Lysophosphatidylcholine	284 564 ± 47 272	256 377 ± 40 459	377 329 ± 48 852
Sphingomyelin	58 827 ± 3 096	55 587 ± 4 108	38 879 ± 4 741**
Phosphatidylcholine	309 929 ± 12 485	305 051 ± 12 172	228 092 ± 7 580***
Liver, pmol/g	n = 5	n = 5	n = 5
Sphingosine	2 261 ± 266	2 064 ± 530	3 290 ± 218*
Sphingosine-1-phosphate	19 ± 4	21 ± 3	37 ± 7 ^P^{= .056}
C16-ceramide	16 201 ± 2 509	12 977 ± 2 015	19 881 ± 1 749
Lysophosphatidylcholine	153 917 ± 20 759	133 929 ± 22 986	152 879 ± 41 454
Sphingomyelin	11 592 ± 2 497	10 591 ± 1 115	16 831 ± 6 129
Phosphatidylcholine	166 124 ± 9 639	182 145 ± 10 753	102 235 ± 19 375*

Sphingolipids and metabolites	HNF1A^+/+	HNF1A^+/−	HNF1A^−/−
Erythrocytes, nM	n = 13	n = 4	n = 7
Sphingosine	5 107 ± 729	7 325 ± 324	9 691 ± 1 328**
C16-ceramide	364 ± 33	438 ± 39	633 ± 39***
Lysophosphatidylcholine	68 985 ± 11 401	65 250 ± 8 128	237 914 ± 86 273*
Sphingomyelin	112 215 ± 14 145	115 150 ± 22 291	67 200 ± 8 990*
Phosphatidylcholine	162 477 ± 20 081	179 450 ± 31 295	139 857 ± 34 142
Plasma, nM	n = 6	n = 6	n = 5
Sphingosine	9 ± 1	11 ± 2	25 ± 1***
Sphingosine-1-phosphate	1 233 ± 56	1 027 ± 55†	772 ± 61***
C16-ceramide	31 ± 5	34 ± 3	35 ± 8
Lysophosphatidylcholine	284 564 ± 47 272	256 377 ± 40 459	377 329 ± 48 852
Sphingomyelin	58 827 ± 3 096	55 587 ± 4 108	38 879 ± 4 741**
Phosphatidylcholine	309 929 ± 12 485	305 051 ± 12 172	228 092 ± 7 580***
Liver, pmol/g	n = 5	n = 5	n = 5
Sphingosine	2 261 ± 266	2 064 ± 530	3 290 ± 218*
Sphingosine-1-phosphate	19 ± 4	21 ± 3	37 ± 7 ^P^{= .056}
C16-ceramide	16 201 ± 2 509	12 977 ± 2 015	19 881 ± 1 749
Lysophosphatidylcholine	153 917 ± 20 759	133 929 ± 22 986	152 879 ± 41 454
Sphingomyelin	11 592 ± 2 497	10 591 ± 1 115	16 831 ± 6 129
Phosphatidylcholine	166 124 ± 9 639	182 145 ± 10 753	102 235 ± 19 375*

Data are presented as mean ± SEM.

P < .05, **P < .01, ***P < .001 HNF1A^+/+ vs HNF1A^−/−, and †P < .05 HNF1A^+/+ vs HNF1A^+/−.