Mass spectrometric analysis of prochemerin 163S cleavage by FXIa, plasmin, and other proteases
| . | Chemerin forms, molecular weight, Da . | |||||
|---|---|---|---|---|---|---|
| 154F . | 155A . | 156F . | 157S . | 158K . | 163S . | |
| Expected molecular weight | 15571.8 | 15642.8 | 15790.0 | 15877.1 | 16005.3 | 16529.9 |
| FXIa | 16019.4 | |||||
| Plasmin | 16003.7 | |||||
| Cathepsin G | 15792.8 | |||||
| Chymase | 15570.0 | 15785.8 | ||||
| Elastase | 15647.3 | 15881.7 | ||||
| Protease 3 | 15648.0 | |||||
| Tryptase | 16011.4 | |||||
| MMP-8 | 15640.4 | |||||
| . | Chemerin forms, molecular weight, Da . | |||||
|---|---|---|---|---|---|---|
| 154F . | 155A . | 156F . | 157S . | 158K . | 163S . | |
| Expected molecular weight | 15571.8 | 15642.8 | 15790.0 | 15877.1 | 16005.3 | 16529.9 |
| FXIa | 16019.4 | |||||
| Plasmin | 16003.7 | |||||
| Cathepsin G | 15792.8 | |||||
| Chymase | 15570.0 | 15785.8 | ||||
| Elastase | 15647.3 | 15881.7 | ||||
| Protease 3 | 15648.0 | |||||
| Tryptase | 16011.4 | |||||
| MMP-8 | 15640.4 | |||||
A total of 10 μM chem163S was incubated for 30 minutes with 100 nM protease before analysis by mass spectrometry. The molecular weight of the observed products was compared with the expected molecular mass.