Hydrolysis of chemerin peptides, FIX peptides, and chemerin full-length protein by FXIa
| Substrate . | Km, μM . | kcat, min–1 . | kcat/Km, M–1 × min–1 . |
|---|---|---|---|
| FIX-10mer | 300 ± 64 | 5.0 ± 0.2 | 1.7 × 104 |
| chem-15mer | 2120 ± 524 | 8.1 ± 1.3 | 3.8 × 103 |
| chem-15merR162A | 1597 ± 230 | 5.5 ± 0.5 | 3.4 × 103 |
| Chem163S | 103 ± 35 | 54.6 ± 5.3 | 5.3 × 108 |
| Substrate . | Km, μM . | kcat, min–1 . | kcat/Km, M–1 × min–1 . |
|---|---|---|---|
| FIX-10mer | 300 ± 64 | 5.0 ± 0.2 | 1.7 × 104 |
| chem-15mer | 2120 ± 524 | 8.1 ± 1.3 | 3.8 × 103 |
| chem-15merR162A | 1597 ± 230 | 5.5 ± 0.5 | 3.4 × 103 |
| Chem163S | 103 ± 35 | 54.6 ± 5.3 | 5.3 × 108 |
Substrates were digested with FXIa as described in “Materials and methods.” The values for Km, kcat, and kcat/Km were compared between peptides derived from chemerin and FIX, as well as chemerin full-length protein. Data are mean ± SEM; n = 3.