Detection of rhTCII in a Sandwich ELISA by Pairs of Anti-TCII Monoclonal Antibodies
| Monoclonal Antibody . | Capturing Antibody . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | 3-11 . | 1-9 . | 3-9 . | 5-18 . | 2-2 . | 2-6 . | 3-5 . | 4-7 . |
| holo-TCII | ||||||||
| 3-11 | − | + | + | + | − | + | + | − |
| 1-9 | +++ | − | ++ | − | +++ | +++ | ++++ | ++++ |
| 3-9 | +++++ | +++++ | − | +++++ | ++++ | − | − | +++++ |
| 5-18 | ++ | − | + | − | ++ | +++ | +++ | +++ |
| 2-2 | − | ++ | + | + | − | + | + | − |
| 2-6 | +++++ | +++++ | − | ++++ | ++++ | − | − | +++++ |
| 3-5 | ++ | ++++ | − | +++ | ++ | − | − | ++++ |
| 4-7 | − | +++++ | ++++ | ++++ | − | +++++ | +++++ | − |
| apo-TCII | ||||||||
| 3-11 | − | + | + | − | − | + | + | − |
| 1-9 | +++ | − | ++ | − | ++ | ++ | +++ | +++ |
| 3-9 | +++++ | ++ | − | + | ++++ | − | − | +++++ |
| 5-18 | ++ | − | ++ | − | ++ | ++ | ++ | ++ |
| 2-2 | − | + | + | − | − | + | + | − |
| 2-6 | +++++ | ++ | − | + | ++++ | − | − | +++++ |
| 3-5 | +++ | ++ | + | − | ++ | − | − | +++ |
| 4-7 | − | ++ | ++++ | + | − | +++++ | +++++ | − |
| Monoclonal Antibody . | Capturing Antibody . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | 3-11 . | 1-9 . | 3-9 . | 5-18 . | 2-2 . | 2-6 . | 3-5 . | 4-7 . |
| holo-TCII | ||||||||
| 3-11 | − | + | + | + | − | + | + | − |
| 1-9 | +++ | − | ++ | − | +++ | +++ | ++++ | ++++ |
| 3-9 | +++++ | +++++ | − | +++++ | ++++ | − | − | +++++ |
| 5-18 | ++ | − | + | − | ++ | +++ | +++ | +++ |
| 2-2 | − | ++ | + | + | − | + | + | − |
| 2-6 | +++++ | +++++ | − | ++++ | ++++ | − | − | +++++ |
| 3-5 | ++ | ++++ | − | +++ | ++ | − | − | ++++ |
| 4-7 | − | +++++ | ++++ | ++++ | − | +++++ | +++++ | − |
| apo-TCII | ||||||||
| 3-11 | − | + | + | − | − | + | + | − |
| 1-9 | +++ | − | ++ | − | ++ | ++ | +++ | +++ |
| 3-9 | +++++ | ++ | − | + | ++++ | − | − | +++++ |
| 5-18 | ++ | − | ++ | − | ++ | ++ | ++ | ++ |
| 2-2 | − | + | + | − | − | + | + | − |
| 2-6 | +++++ | ++ | − | + | ++++ | − | − | +++++ |
| 3-5 | +++ | ++ | + | − | ++ | − | − | +++ |
| 4-7 | − | ++ | ++++ | + | − | +++++ | +++++ | − |
Pairs of anti-TCII monoclonal antibodies were tested for binding to recombinant human holo-TCII and recombinant human apo-TCII. Data are expressed in +/− format, where + represents approximately 0.3 OD units and − represents background OD.