LTRSC Maintained on AFT024 Are Able to Repopulate Secondary Recipients at Levels Comparable to Noncultured Stem Cells
| Group . | Week* . | % Ly5.2+ Peripheral Blood Cells . | |
|---|---|---|---|
| . | . | Radioprotection . | Competitive Repopulation . |
| Control | 6 | 21 (1) | 2.6 ± 0.6 (4) |
| 22 | 54 (1) | 5.6 ± 1.6 (4) | |
| AFT024 | 6 | 13 ± 0.2 (4) | 1.3 ± 0.9 (8) |
| 22 | 44 ± 15.1 (3) | 4.3 ± 0.9 (8) | |
| 2012 | 6 | 6.7 ± 2.9 (4) | ND |
| 22 | 14 ± 2.2 (4) | ND | |
| Group . | Week* . | % Ly5.2+ Peripheral Blood Cells . | |
|---|---|---|---|
| . | . | Radioprotection . | Competitive Repopulation . |
| Control | 6 | 21 (1) | 2.6 ± 0.6 (4) |
| 22 | 54 (1) | 5.6 ± 1.6 (4) | |
| AFT024 | 6 | 13 ± 0.2 (4) | 1.3 ± 0.9 (8) |
| 22 | 44 ± 15.1 (3) | 4.3 ± 0.9 (8) | |
| 2012 | 6 | 6.7 ± 2.9 (4) | ND |
| 22 | 14 ± 2.2 (4) | ND | |
The retransplantation potential of LTRSC in primary recipients of stromal cell cultured stem cells was studied in secondary recipients. 60 weeks after transplant, primary mice (Fig 1, Table 1) were killed, and BM was harvested and stained with antibody to Ly5.2. Ly5.2+ cells were collected by cell sorting and used to transplant secondary recipients (congenic Ly5.1 mice). Control and AFT024 groups were transplanted with 1.5 × 106 Ly5.2 cells per mouse for radioprotection (4 mice/group were transplanted) and 7.5 × 105 Ly5.2 cells + 7.5 × 105 Ly5.1 cells for competitive repopulation (4 mice for the control group and 8 mice for the AFT024 group). 2012 mice were transplanted with 3 × 105 Ly5.2 cells per mouse (4 mice). Number in parentheses is the number of mice surviving per group. ND, not determined. Data are the mean ± SEM.
Time after transplant that the mice were analyzed.