Summary of the Effects of Various Pharmacologic Interventions on Eosinophil Aggregation, Intracellular Ca2+ Transients, and Recruitment In Vivo Induced by PAF, C5a, and LTB4
| . | PAF . | C5a . | LTB4 . |
|---|---|---|---|
| PTX (1 μg/mL) | |||
| Ca2+ transient | Unaffected | Abolished | Attenuated by ∼90% |
| Aggregation | Unaffected | Abolished | Attenuated by ∼75% |
| In vivo recruitment | Attenuated by ∼50% | Abolished | AbolishedRo 31-8220 (3 × 10−6 mol/L) |
| Ca2+ transient | Abolished | Prolonged, no change in amplitude | Prolonged, no change in amplitude |
| Aggregation | Abolished | Unaffected or enhanced | Inhibited at low concentrations Stimulated at high concentrationsStaurosporine (10−7 mol/L) |
| Ca2+ transient | Unaffected | Prolonged, no change in amplitude | Prolonged, no change in amplitude |
| Aggregation | Enhanced | Enhanced | EnhancedH-89 (10−5 mol/L) |
| Aggregation | Enhanced | Enhanced | Enhanced |
| . | PAF . | C5a . | LTB4 . |
|---|---|---|---|
| PTX (1 μg/mL) | |||
| Ca2+ transient | Unaffected | Abolished | Attenuated by ∼90% |
| Aggregation | Unaffected | Abolished | Attenuated by ∼75% |
| In vivo recruitment | Attenuated by ∼50% | Abolished | AbolishedRo 31-8220 (3 × 10−6 mol/L) |
| Ca2+ transient | Abolished | Prolonged, no change in amplitude | Prolonged, no change in amplitude |
| Aggregation | Abolished | Unaffected or enhanced | Inhibited at low concentrations Stimulated at high concentrationsStaurosporine (10−7 mol/L) |
| Ca2+ transient | Unaffected | Prolonged, no change in amplitude | Prolonged, no change in amplitude |
| Aggregation | Enhanced | Enhanced | EnhancedH-89 (10−5 mol/L) |
| Aggregation | Enhanced | Enhanced | Enhanced |