Table 2. p21 Waf1 and p16 INK4a Gene... | American Society of Hematology

Table 2.

p21^Waf1 and p16^INK4a Gene Alterations and Expression in Aggressive MCLs

Case	Histology	p53	p21^Waf1
			Gene		Expresssion		p16^INK4a
			SB	SSCP/Sequence	mRNA	Protein (WB and IHQ)	Gene		Expression
							SB	SSCP/Sequence	mRNA	Protein
3858	Blastic	WT	+/+	WT	+	+	−/−	NA	−	−
15566	Blastic	WT	+/+	WT	ND	+	−/−	NA	ND	−
3747	Large cell	WT	ND	ND	+	+^*	+/−^†	ND	ND	ND
441	Blastic	WT	+/+	WT	−	−	+/+	WT	+	++
1104	Large cell	M Codon 273	+/+	WT	+	+	+/+	WT	++	++
4958	Blastic	M Codon 278	+/+	WT	+	+	+/+	WT	+	ND

Case	Histology	p53	p21^Waf1
			Gene		Expresssion		p16^INK4a
			SB	SSCP/Sequence	mRNA	Protein (WB and IHQ)	Gene		Expression
							SB	SSCP/Sequence	mRNA	Protein
3858	Blastic	WT	+/+	WT	+	+	−/−	NA	−	−
15566	Blastic	WT	+/+	WT	ND	+	−/−	NA	ND	−
3747	Large cell	WT	ND	ND	+	+^*	+/−^†	ND	ND	ND
441	Blastic	WT	+/+	WT	−	−	+/+	WT	+	++
1104	Large cell	M Codon 273	+/+	WT	+	+	+/+	WT	++	++
4958	Blastic	M Codon 278	+/+	WT	+	+	+/+	WT	+	ND

Abbreviations: SB, Southern blotting; WB, Western blot; IHQ, immunohistochemistry; SB: +/+ no deletion; +/− hemizygous deletion; −/− homozygous deletion; M, mutated; WT, wild-type; ND, not done; NA, not applicable.

In this case protein p21^Waf1 protein expression was only detected by immunohistochemistry.

†

In this case a hemizygous deletion of 9p arm −del (9) (p12)− was detected by cytogenetic analysis. No DNA was available for Southern blotting.

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