Gene Marking Frequency
| . | . | Transduction4-150 . | ||
|---|---|---|---|---|
| . | . | LIF . | LIF [+AZT]4-151 . | SCF . |
| CoH-1 | CFU-C | 4 (4/90) | 1 (1/78) | 8 (7/90) |
| LTC-CFC | 2 (2/90) | 2 (2/92) | 2 (2/90) | |
| CoH-2 | CFU-C | 3 (3/91) | 2 (2/91) | 2 (2/91) |
| LTC-CFC | 4 (4/92) | 3 (3/90) | 3 (3/86) | |
| CoH-3 | CFU-C | 4 (3/84) | 2 (2/91) | 6 (5/90) |
| LTC-CFC | 6 (5/88) | 2 (2/92) | 1 (1/91) | |
| . | . | Transduction4-150 . | ||
|---|---|---|---|---|
| . | . | LIF . | LIF [+AZT]4-151 . | SCF . |
| CoH-1 | CFU-C | 4 (4/90) | 1 (1/78) | 8 (7/90) |
| LTC-CFC | 2 (2/90) | 2 (2/92) | 2 (2/90) | |
| CoH-2 | CFU-C | 3 (3/91) | 2 (2/91) | 2 (2/91) |
| LTC-CFC | 4 (4/92) | 3 (3/90) | 3 (3/86) | |
| CoH-3 | CFU-C | 4 (3/84) | 2 (2/91) | 6 (5/90) |
| LTC-CFC | 6 (5/88) | 2 (2/92) | 1 (1/91) | |
Retroviral gene marking, determined by neo (neomycin phosphotransferase) gene DNA PCR, is expressed as a percentage and the number of neo-positive colonies divided by the total number of β-globin–positive colonies is indicated in parentheses.
Supernatant transduction was performed in the presence of IL-3, IL-6, and LIF (LIF) or IL-3, IL-6, and SCF (SCF). For details, see the Materials and Methods.
Cells were plated after transduction in methylcellulose (CFU-C) or cultured on Sys1 mouse stromal cells (LTC-CFC) in the presence of AZT.