Selectivity of Cell Migration Induced by Plasmin
| Group . | No. of Migrated Cells . | |
|---|---|---|
| . | PM . | PMN . |
| Control | 5.5 ± 0.4 | 5.0 ± 2.5 |
| FMLP (10 nmol/L) | 21.5 ± 1.2* | 20.3 ± 0.2* |
| Plasmin (CTA U/mL) | ||
| 2.86 | 16.5 ± 1.8* | — |
| 1.43 | 21.1 ± 0.9* | 3.1 ± 1.4 |
| 0.43 | 20.5 ± 0.9* | 4.6 ± 1.5 |
| 0.14 | 15.3 ± 1.0* | 3.4 ± 1.6 |
| 0.04 | 10.8 ± 0.9* | 3.4 ± 1.7 |
| 0.01 | 9.3 ± 0.6* | 5.0 ± 2.1 |
| Group . | No. of Migrated Cells . | |
|---|---|---|
| . | PM . | PMN . |
| Control | 5.5 ± 0.4 | 5.0 ± 2.5 |
| FMLP (10 nmol/L) | 21.5 ± 1.2* | 20.3 ± 0.2* |
| Plasmin (CTA U/mL) | ||
| 2.86 | 16.5 ± 1.8* | — |
| 1.43 | 21.1 ± 0.9* | 3.1 ± 1.4 |
| 0.43 | 20.5 ± 0.9* | 4.6 ± 1.5 |
| 0.14 | 15.3 ± 1.0* | 3.4 ± 1.6 |
| 0.04 | 10.8 ± 0.9* | 3.4 ± 1.7 |
| 0.01 | 9.3 ± 0.6* | 5.0 ± 2.1 |
Different dilutions of plasmin were added to the lower compartments of the Transwell chambers. Human monocytes (PMs) were permitted to migrate across polycarbonate membranes with a pore size of 5 μm for 90 minutes. Human PMNs were allowed to migrate across polycarbonate membranes with a pore size of 3 μm for 60 minutes. Results represent the mean ± SEM for migrated cells in 7 oil-immersion fields of 9 PM or 3 PMN experiments.
P < .01 v HBSS-BSA 0.4% controls.