Checkerboard Analysis of Monocyte Migration Across Polycarbonate Membranes After Stimulation With Plasmin
| Below . | Above . | |||||
|---|---|---|---|---|---|---|
| . | HBSS . | Plasmin (CTA U/mL) . | . | . | ||
| . | . | 0.04 . | 0.14 . | 0.43 . | . | . |
| HBSS | 4.8 ± 0.6 | 5.7 ± 0.5 | 5.2 ± 0.7 | 4.8 ± 0.7 | ||
| Plasmin (CTA U/mL) | ||||||
| 0.04 | 9.7 ± 1.2 | 6.4 ± 0.8 | 6.0 ± 0.6 | 5.2 ± 0.7 | ||
| 0.14 | 14.3 ± 1.0 | 8.5 ± 0.9 | 6.9 ± 0.7 | 4.6 ± 0.6 | ||
| 0.43 | 19.8 ± 1.0 | 11.1 ± 0.7 | 7.2 ± 0.7 | 6.8 ± 0.8 | ||
| Below . | Above . | |||||
|---|---|---|---|---|---|---|
| . | HBSS . | Plasmin (CTA U/mL) . | . | . | ||
| . | . | 0.04 . | 0.14 . | 0.43 . | . | . |
| HBSS | 4.8 ± 0.6 | 5.7 ± 0.5 | 5.2 ± 0.7 | 4.8 ± 0.7 | ||
| Plasmin (CTA U/mL) | ||||||
| 0.04 | 9.7 ± 1.2 | 6.4 ± 0.8 | 6.0 ± 0.6 | 5.2 ± 0.7 | ||
| 0.14 | 14.3 ± 1.0 | 8.5 ± 0.9 | 6.9 ± 0.7 | 4.6 ± 0.6 | ||
| 0.43 | 19.8 ± 1.0 | 11.1 ± 0.7 | 7.2 ± 0.7 | 6.8 ± 0.8 | ||
Monocytes were permitted to migrate for 90 minutes across polycarbonate membranes with a pore size of 5 μm. Different concentrations of plasmin were placed in the upper and/or lower compartments of the Transwell chambers. Results represent the mean ± SEM for migrated monocytes in 7 oil-immersion fields counted in triplicate from 4 experiments.