Table 1.

Comparison of the Abilities of Different Strains of Sublethally Irradiated Immunodeficient Mice to be Engrafted With Human Marrow Cells

Recipient GenotypeNo. of Human Cells Injected (per mouse)Dose of Radiation Given to RecipientsMethod Used to Detect EngraftmentNo. of Positive Mice/No. of Mice Transplanted (no. of experiments)
NOD/SCID 107 360 FACS* 10/10 (4) 
 2 × 107 400 Southern† 11/11 (3) 
CB17/SCID 107 360 FACS 0/10 (4) 
 2 × 107 400 Southern 2/3 (1) 
BEIGE/SCID 107 360 FACS 0/7 (3) 
RAG-2 −/− 107 1200 FACS 0/10 (4) 
 (2-4) × 107 700-800 Southern 0/5 (2) 
Recipient GenotypeNo. of Human Cells Injected (per mouse)Dose of Radiation Given to RecipientsMethod Used to Detect EngraftmentNo. of Positive Mice/No. of Mice Transplanted (no. of experiments)
NOD/SCID 107 360 FACS* 10/10 (4) 
 2 × 107 400 Southern† 11/11 (3) 
CB17/SCID 107 360 FACS 0/10 (4) 
 2 × 107 400 Southern 2/3 (1) 
BEIGE/SCID 107 360 FACS 0/7 (3) 
RAG-2 −/− 107 1200 FACS 0/10 (4) 
 (2-4) × 107 700-800 Southern 0/5 (2) 
*

Positivity = >1% of viable cells detected in the recipient BM at either 7 or 10 weeks posttransplant were human CD45+ and/or human CD71+.

Positivity = >0.1% human DNA as indicated by probing the Southern blot with a human-specific α-satellite probe.

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