Comparison of the Abilities of Different Strains of Sublethally Irradiated Immunodeficient Mice to be Engrafted With Human Marrow Cells
Recipient Genotype . | No. of Human Cells Injected (per mouse) . | Dose of Radiation Given to Recipients . | Method Used to Detect Engraftment . | No. of Positive Mice/No. of Mice Transplanted (no. of experiments) . |
---|---|---|---|---|
NOD/SCID | 107 | 360 | FACS* | 10/10 (4) |
2 × 107 | 400 | Southern† | 11/11 (3) | |
CB17/SCID | 107 | 360 | FACS | 0/10 (4) |
2 × 107 | 400 | Southern | 2/3 (1) | |
BEIGE/SCID | 107 | 360 | FACS | 0/7 (3) |
RAG-2 −/− | 107 | 1200 | FACS | 0/10 (4) |
(2-4) × 107 | 700-800 | Southern | 0/5 (2) |
Recipient Genotype . | No. of Human Cells Injected (per mouse) . | Dose of Radiation Given to Recipients . | Method Used to Detect Engraftment . | No. of Positive Mice/No. of Mice Transplanted (no. of experiments) . |
---|---|---|---|---|
NOD/SCID | 107 | 360 | FACS* | 10/10 (4) |
2 × 107 | 400 | Southern† | 11/11 (3) | |
CB17/SCID | 107 | 360 | FACS | 0/10 (4) |
2 × 107 | 400 | Southern | 2/3 (1) | |
BEIGE/SCID | 107 | 360 | FACS | 0/7 (3) |
RAG-2 −/− | 107 | 1200 | FACS | 0/10 (4) |
(2-4) × 107 | 700-800 | Southern | 0/5 (2) |