Engraftment of Rh/Hoedull Marrow Cells in Nonablated Recipients
| No. Donor Cells . | Cloning Efficiency %* . | No. of Recipients . | % Male in Whole Marrow Using Southern Blot Analysis† . | % Male Cells in Whole Marrow Determined Using FISH‡ . |
|---|---|---|---|---|
| Rh/Hoedull | ||||
| 2,600ρ | 82.3 | 12 | 1.4 ± 0.2 | 1.7 ± 0.2 |
| 3,0001-155 | 88.7 | 9 | 0.9 ± 0.07 | 1.1 ± 0.2 |
| 5,500ρ | 49.8 | 3 | 1.2 ± 0.4 | 1.0 ± 0.3 |
| 10,000ρ | 86.7 | 3 | 0.7 ± 0.4 | ND |
| 10,000ρ | 81.3 | 5 | 2.2 ± 0.4 | 3.4 ± 0.4 |
| Whole marrow | ||||
| 100 × 106ρ | ND | 23 | 24.9 ± 1.9 | ND |
| No. Donor Cells . | Cloning Efficiency %* . | No. of Recipients . | % Male in Whole Marrow Using Southern Blot Analysis† . | % Male Cells in Whole Marrow Determined Using FISH‡ . |
|---|---|---|---|---|
| Rh/Hoedull | ||||
| 2,600ρ | 82.3 | 12 | 1.4 ± 0.2 | 1.7 ± 0.2 |
| 3,0001-155 | 88.7 | 9 | 0.9 ± 0.07 | 1.1 ± 0.2 |
| 5,500ρ | 49.8 | 3 | 1.2 ± 0.4 | 1.0 ± 0.3 |
| 10,000ρ | 86.7 | 3 | 0.7 ± 0.4 | ND |
| 10,000ρ | 81.3 | 5 | 2.2 ± 0.4 | 3.4 ± 0.4 |
| Whole marrow | ||||
| 100 × 106ρ | ND | 23 | 24.9 ± 1.9 | ND |
Abbreviation: ND, not done.
The cloning efficiency represents the proportion of 7 factor (IL-1, IL-3, CSF-1, GM-CSF, G-CSF, SCF, and FGF), responsive HPP-CFC after 14 days in a double-layer agar culture.
Values are the means ± SEM. Southern blots were probed with pY2 and the percentage male was determined using phosphorimage analysis, taking the male to be 100% and the female as 0%. Loading variability was corrected using an IL-3 probe.
Values are the means ± SEM representing analysis of at least 300 cells from at least 4 different fields of focus. FISH analysis was done using a Y chromosome-specific painting probe. Male slides were 100%, and female slides 0% following this analysis.
ρ Analyzed 6 weeks posttransplant.
Analyzed 6 months posttransplant.