Analysis of Multilineage Potential
| . | % Subpopulation in Peripheral WBC* . | % Male in Each Subpopulation† . |
|---|---|---|
| MAC-1 | 13.8 ± 2.7 | 0.4 ± 0.2 |
| GR-1 | 7.5 ± 0.6 | 1.0 ± 0.6 |
| B220 | 16.1 ± 3.1 | 2.2 ± 0.6 |
| CD4/CD8 | 24.3 ± 2.0 | 0.3 ± 0.3 |
| . | % Subpopulation in Peripheral WBC* . | % Male in Each Subpopulation† . |
|---|---|---|
| MAC-1 | 13.8 ± 2.7 | 0.4 ± 0.2 |
| GR-1 | 7.5 ± 0.6 | 1.0 ± 0.6 |
| B220 | 16.1 ± 3.1 | 2.2 ± 0.6 |
| CD4/CD8 | 24.3 ± 2.0 | 0.3 ± 0.3 |
Mice were injected with 5,500 Rh/Hoedull cells and harvested 6 weeks posttransplant. The percentage donor cells in the BM as determined by FISH was 1.0 ± 0.3%. Peripheral blood was collected, and the red blood cells lysed using ammonium chloride.
White cells were labeled for macrophages (MAC-1), granulocytes (GR-1), B cells (B220), and T cells (CD4/CD8).
Each peripheral white blood cell (WBC) subpopulation was analyzed for donor cells by FISH using a Y chromosome-specific painting probe. Values are the means ± SEM.