Isolation and Phenotype of Primary HIV Isolates Obtained From Plasma, PBMCs, and CD8-Depleted PBMCs of LTNP and Progressor Hemophiliacs
| Patient No. . | Plasma . | PBMCs . | CD8-Depleted PBMCs . | |||
|---|---|---|---|---|---|---|
| . | RT . | p24 Ag . | RT . | p24 Ag . | RT . | p24 Ag . |
| LTNPs | ||||||
| 1 | — | — | — | — | — | — |
| 2 | — | — | SI | |||
| 3 | — | — | — | NSI | ||
| 4 | — | — | — | — | — | — |
| 5 | — | — | — | — | NSI | |
| 6 | — | — | NSI | |||
| 7 | — | — | — | — | — | NSI |
| Progressors | ||||||
| 8 | NSI | NSI | ||||
| 9 | — | — | NSI | |||
| 10 | — | — | NSI | |||
| 11 | NSI | NSI | ||||
| 12 | NSI | SI | ||||
| 13 | NSI | SI | ||||
| 14 | SI | SI | ||||
| 15 | NSI | SI | ||||
| Patient No. . | Plasma . | PBMCs . | CD8-Depleted PBMCs . | |||
|---|---|---|---|---|---|---|
| . | RT . | p24 Ag . | RT . | p24 Ag . | RT . | p24 Ag . |
| LTNPs | ||||||
| 1 | — | — | — | — | — | — |
| 2 | — | — | SI | |||
| 3 | — | — | — | NSI | ||
| 4 | — | — | — | — | — | — |
| 5 | — | — | — | — | NSI | |
| 6 | — | — | NSI | |||
| 7 | — | — | — | — | — | NSI |
| Progressors | ||||||
| 8 | NSI | NSI | ||||
| 9 | — | — | NSI | |||
| 10 | — | — | NSI | |||
| 11 | NSI | NSI | ||||
| 12 | NSI | SI | ||||
| 13 | NSI | SI | ||||
| 14 | SI | SI | ||||
| 15 | NSI | SI | ||||
Viral isolation was attempted by cocultivation of either plasma or cells from HIV-infected individuals with a mixture of PHA blasts of 2 independent donors. Viral phenotype observed by cultivation of MT-2 cells with HIV isolates.32