Table 1. Effects of IFN-γ, Melittin, and... | American Society of Hematology

Table 1.

Effects of IFN-γ, Melittin, and Different Inhibitors on AA Release by HL-60 Cells

Treatment	Control	IFN-γ
None	100 ± 13	661 ± 56^*
Melittin	561 ± 42^*	780 ± 67^*
BPB	30 ± 7^†	33 ± 3^†
Indomethacin	117 ± 14	590 ± 36^*
NDGA	113 ± 19	617 ± 53^*
Tyrphostine	112 ± 15	638 ± 26^*
Pertussis toxin	106 ± 15	113 ± 17

Treatment	Control	IFN-γ
None	100 ± 13	661 ± 56^*
Melittin	561 ± 42^*	780 ± 67^*
BPB	30 ± 7^†	33 ± 3^†
Indomethacin	117 ± 14	590 ± 36^*
NDGA	113 ± 19	617 ± 53^*
Tyrphostine	112 ± 15	638 ± 26^*
Pertussis toxin	106 ± 15	113 ± 17

Cells were labeled with 5 μCi/mL of [³H]arachidonic acid for 60 minutes, washed, and treated with IFN-γ (2,000 U/mL), melittin (500 ng/mL) or the combination of IFN-γ (2,000 U/mL) and melittin (500 ng/mL) for 2 minutes. After pretreatment with BPB, 10 μmol/L for 30 minutes, indomethacin (50 μmol/L) for 15 minutes, NDGA (50 μmol/L) for 15 minutes, tyrphostine (20 μmol/L) for 60 minutes, and pertussis toxin (20 ng/mL) for 18 hours, cells were stimulated or not with 2,000 U/mL of IFN-γ for 2 minutes. After incubation, cells were pelleted and the resultant supernatant was counted to determine levels of released label. The data (means ± SD) are expressed as percentage of the control level obtained from three independent experiments assayed in duplicate.

Significantly different (P < .05, Student's t-test) from controls.

†

Significantly different from both untreated and IFN-γ–stimulated cells.

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