Cytochemical Staining of AChE in MK From Normal and ITP BM
| Cytochemical AChE Activity . | MK Size . | ||
|---|---|---|---|
| . | Small <10 μm . | Intermediate 10-25 μm . | Large >25 μm . |
| Normal (TPO-stimulated) | |||
| ++ | 93.3 | 37.5 | 2.8 |
| + | 5.7 | 42.7 | 14.3 |
| − | 1.0 | 19.8 | 82.9 |
| Normal BM smear | |||
| ++ | 68.2 | 12.8 | 2.2 |
| + | 26.3 | 42.3 | 16.2 |
| − | 5.5 | 44.9 | 81.6 |
| ITP | |||
| ++ | 98.3 | 50.5 | 5.2 |
| + | 0 | 34.0 | 32.5 |
| − | 1.7 | 15.5 | 62.3 |
| Cytochemical AChE Activity . | MK Size . | ||
|---|---|---|---|
| . | Small <10 μm . | Intermediate 10-25 μm . | Large >25 μm . |
| Normal (TPO-stimulated) | |||
| ++ | 93.3 | 37.5 | 2.8 |
| + | 5.7 | 42.7 | 14.3 |
| − | 1.0 | 19.8 | 82.9 |
| Normal BM smear | |||
| ++ | 68.2 | 12.8 | 2.2 |
| + | 26.3 | 42.3 | 16.2 |
| − | 5.5 | 44.9 | 81.6 |
| ITP | |||
| ++ | 98.3 | 50.5 | 5.2 |
| + | 0 | 34.0 | 32.5 |
| − | 1.7 | 15.5 | 62.3 |
More than 400 MK were counted in each group and the cells were classified according to their size into small, intermediate, and large categories as in Fig 6. Arbitrary degrees of AChE staining determined under ×1,000 magnification were defined as ++ (intensely and moderately stained), + (weakly stained), or − (not stained at all). Data are presented as the percentage of cells counted in each category. The presented analysis was performed on 1 of 3 normal or ITP patients and 1 of 5 TPO-expanded normal BM cultures.