Cytotoxicity of T-Cell Clones Against CD34+ Cells
| Target . | Effector . | |
|---|---|---|
| . | NT4.2 . | NT27 . |
| A | ||
| Freshly obtained autologous CD34+ cells | 2.4 | 2.1 |
| Cultured autologous CD34+ cells | 14.8 | 3.2 |
| B | ||
| Cultured allogeneic CD34+ cells from patient A | 10.2 | NT |
| Cultured allogeneic CD34+ cells from patient B | 2.8 | NT |
| Cultured allogeneic CD34+ cells from patient C | 3.1 | NT |
| Target . | Effector . | |
|---|---|---|
| . | NT4.2 . | NT27 . |
| A | ||
| Freshly obtained autologous CD34+ cells | 2.4 | 2.1 |
| Cultured autologous CD34+ cells | 14.8 | 3.2 |
| B | ||
| Cultured allogeneic CD34+ cells from patient A | 10.2 | NT |
| Cultured allogeneic CD34+ cells from patient B | 2.8 | NT |
| Cultured allogeneic CD34+ cells from patient C | 3.1 | NT |
Specific lysis of CD34+ cells from the patient and normal individuals (patient A, HLA-DRB1*0405, 0901; patient B, HLA-DRB1*0901, 0803; patient C, HLA-DRB1*0803, 1302) by cloned T cells was measured using a 51Cr release assay in triplicate at effector/target ratio 10:1. Data are expressed as mean percentage specific lysis.
Abbreviation: NT, not tested.