Table 2. Effect of T-Cell Clones on... | American Society of Hematology

Table 2.

Effect of T-Cell Clones on Autologous Hematopoietic Progenitor Cell Growth

Cells Plated for Progenitor Cell Assay	T-Cell Clone Added	Preincubation	CFU-GM	BFU-E
Experiment 1
Freshly obtained CD34⁺ cells	—	−	232 ± 17	192 ± 12
	NT4.2	−	194 ± 25	152 ± 16
	NT4.2	+	201 ± 12	144 ± 16
	NT27	+	194 ± 19	166 ± 11
Cultured CD34⁺ cells	—	−	98 ± 13	7 ± 2
	NT4.2	−	74 ± 6	6 ± 2
	NT4.2	+	2 ± 1	1 ± 1
	NT27	+	78 ± 4	8 ± 2
Experiment 2
Cryopreserved-thawed CD34⁺ cells^*	—	−	92 ± 6	40 ± 3
	NT4.2	−	80 ± 5	34 ± 3
	NT4.2	+	81 ± 5	31 ± 5
Cultured CD34⁺ cells	—	−	78 ± 6	19 ± 3
	NT4.2	−	70 ± 2	15 ± 1
	NT4.2	+	3 ± 1	0
	NT4.2 + DR MoAb^†	+	40 ± 7	6 ± 1

Cells Plated for Progenitor Cell Assay	T-Cell Clone Added	Preincubation	CFU-GM	BFU-E
Experiment 1
Freshly obtained CD34⁺ cells	—	−	232 ± 17	192 ± 12
	NT4.2	−	194 ± 25	152 ± 16
	NT4.2	+	201 ± 12	144 ± 16
	NT27	+	194 ± 19	166 ± 11
Cultured CD34⁺ cells	—	−	98 ± 13	7 ± 2
	NT4.2	−	74 ± 6	6 ± 2
	NT4.2	+	2 ± 1	1 ± 1
	NT27	+	78 ± 4	8 ± 2
Experiment 2
Cryopreserved-thawed CD34⁺ cells^*	—	−	92 ± 6	40 ± 3
	NT4.2	−	80 ± 5	34 ± 3
	NT4.2	+	81 ± 5	31 ± 5
Cultured CD34⁺ cells	—	−	78 ± 6	19 ± 3
	NT4.2	−	70 ± 2	15 ± 1
	NT4.2	+	3 ± 1	0
	NT4.2 + DR MoAb^†	+	40 ± 7	6 ± 1

A total of 3 × 10³ freshly isolated CD34⁺ cells or CD34⁺ cells cultured in the presence of colony-stimulating factors were incubated in medium alone or with 3 × 10⁴ cloned T cells for 4 hours (preincubation +) and then mixed with methylcellulose medium supplemented with growth factors. Some CD34⁺ cells were mixed with cloned T cells just before addition of the methylcellulose medium (preincubation −). Data are expressed as mean ± standard error of duplicate cultures.

An aliquot of autologous CD34⁺ cells that had been cryopreserved was used in Experiment 2.

†

Anti–HLA-DR MoAb, ascites 1/100, was added to the culture of CD34⁺ cells with NT4.2.

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