Effect of T-Cell Clones on Colony Formation by Allogeneic CD34+ Cells
| Donor of CD34+ Cells . | Preincubation . | . | CFU-GM . | BFU-E . |
|---|---|---|---|---|
| A | Control | 168 ± 12 | 79 ± 6 | |
| − | 142 ± 9 | 69 ± 4 | ||
| + | 40 ± 5 | 9 ± 2 | ||
| B | Control | 106 ± 7 | 73 ± 5 | |
| − | 99 ± 6 | 70 ± 6 | ||
| + | 80 ± 5 | 66 ± 4 | ||
| C | Control | 184 ± 12 | 91 ± 7 | |
| − | 168 ± 10 | 88 ± 3 | ||
| + | 160 ± 7 | 78 ± 5 |
| Donor of CD34+ Cells . | Preincubation . | . | CFU-GM . | BFU-E . |
|---|---|---|---|---|
| A | Control | 168 ± 12 | 79 ± 6 | |
| − | 142 ± 9 | 69 ± 4 | ||
| + | 40 ± 5 | 9 ± 2 | ||
| B | Control | 106 ± 7 | 73 ± 5 | |
| − | 99 ± 6 | 70 ± 6 | ||
| + | 80 ± 5 | 66 ± 4 | ||
| C | Control | 184 ± 12 | 91 ± 7 | |
| − | 168 ± 10 | 88 ± 3 | ||
| + | 160 ± 7 | 78 ± 5 |
A total of 3 × 103 allogeneic CD34+ cells cultured in the presence of colony-stimulating factors were incubated in medium with (preincubation +) or without (control) 3 × 104 NT4.2 cells for 4 hours and then mixed with methylcellulose medium for colony assay. In some experiments, CD34+ cells were mixed with NT4.2 cells just before addition to methylcellulose medium (preincubation −). Data are expressed as mean ± standard error of duplicate cultures. Patient A, HLA-DRB1*0405, 0901; patient B, HLA-DRB1*0901, 0803; patient C, HLA-DRB1*0803, 1302.