Neutrophil Respiratory Burst Activity and Intracellular GSH After Fas Incubation
| . | Control . | Anti-Fas IgM (100 ng/mL) . | ||
|---|---|---|---|---|
| . | . | 30 Min . | 60 Min . | 120 Min . |
| Reactive oxygen intermediates (LnMCF) | 15.2 ± 11.0 | 14.9 ± 4.9 | 12.1 ± 5.0 | 15.6 ± 8.7 |
| Chemiluminescence counts per PMN | 18.3 ± 8.2 | 24.1 ± 10.6 | 20.3 ± 9.1 | 19.0 ± 10.0 |
| GSH (nmols/10−7 PMN) | 8.3 ± 0.5 | — | 9.1 ± 1.2 | — |
| . | Control . | Anti-Fas IgM (100 ng/mL) . | ||
|---|---|---|---|---|
| . | . | 30 Min . | 60 Min . | 120 Min . |
| Reactive oxygen intermediates (LnMCF) | 15.2 ± 11.0 | 14.9 ± 4.9 | 12.1 ± 5.0 | 15.6 ± 8.7 |
| Chemiluminescence counts per PMN | 18.3 ± 8.2 | 24.1 ± 10.6 | 20.3 ± 9.1 | 19.0 ± 10.0 |
| GSH (nmols/10−7 PMN) | 8.3 ± 0.5 | — | 9.1 ± 1.2 | — |
Neutrophils were incubated with anti-Fas IgM (CH-11, 100 ng/mL) and then assessed for reactive oxygen intermediates after 0, 30, 60, and 120 minutes by flow cytometry using dihydrorhodamine 123 or neutrophil induced chemiluminescence. Intracellular GSH was measured at 60 minutes. Results represent the mean ± SD of n = 3 experiments.