Effect of CD31 Peptide on the Secondary MLR
| Restimulator . | Primed Balb/c Cells in the Absence of CD31 Peptide . | Primed Balb/c Cells in the Presence of CD31 Peptide . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Culture Days and cpm . | 3 . | 4 . | 5 . | 6 . | 7 . | Culture Days and cpm . | 3 . | 4 . | 5 . | 6 . | 7 . |
| . | 2 . | . | . | . | . | . | 2 . | . | . | . | . | . |
| None | 1,251 | 779 | 549 | 462 | 388 | 307 | 1,955 | 1,701 | 1,288 | 1,021 | 557 | 560 |
| C57BL/6 | 207,504 | 192,084 | 97,668 | 54,524 | 63,116 | 63,116 | 2,001 | 1,904 | 1,474 | 1,280 | 760 | 572 |
| CBA | 37,294 | 34,634 | 48,541 | 40,068 | 54,919 | 47,958 | 2,560 | 2,130 | 1,740 | 1,286 | 685 | 655 |
| C57BL/6 + IL-2 | — | — | — | — | — | — | 2,608 | 3,920 | 1,626 | 1,208 | 1,328 | 1,021 |
| ConA | 76,783 | — | — | — | — | — | 26,464 | — | — | — | — | — |
| Restimulator . | Primed Balb/c Cells in the Absence of CD31 Peptide . | Primed Balb/c Cells in the Presence of CD31 Peptide . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Culture Days and cpm . | 3 . | 4 . | 5 . | 6 . | 7 . | Culture Days and cpm . | 3 . | 4 . | 5 . | 6 . | 7 . |
| . | 2 . | . | . | . | . | . | 2 . | . | . | . | . | . |
| None | 1,251 | 779 | 549 | 462 | 388 | 307 | 1,955 | 1,701 | 1,288 | 1,021 | 557 | 560 |
| C57BL/6 | 207,504 | 192,084 | 97,668 | 54,524 | 63,116 | 63,116 | 2,001 | 1,904 | 1,474 | 1,280 | 760 | 572 |
| CBA | 37,294 | 34,634 | 48,541 | 40,068 | 54,919 | 47,958 | 2,560 | 2,130 | 1,740 | 1,286 | 685 | 655 |
| C57BL/6 + IL-2 | — | — | — | — | — | — | 2,608 | 3,920 | 1,626 | 1,208 | 1,328 | 1,021 |
| ConA | 76,783 | — | — | — | — | — | 26,464 | — | — | — | — | — |
A total of 5 × 106 BALB/c spleen cells was primed with 5 × 106 C57BL/6 irradiated spleen cells in absence of peptide (left) or presence of CD31 peptide (right) or control peptide (20 μg/mL). Primed cells were restimulated with fresh stimulator C57BL/6 cells or CBA cells or ConA 2 μg/mL and also IL-2 200 IU per well was added to the additional wells, where primed cells were restimulated with irradiated BALB/c stimulator. Results are expressed as cpm from triplicate cultures. Typical standard deviations were <20% of the mean. Cells primed in the presence of control peptide showed the similar results to cells primed without peptide (data not shown).