Flow Cytometric Analysis of K562 Transfectants Expressing Full-Length and Domain-Deleted Lu cDNA
| K562 Cells Transfected With cDNA Constructs . | Monoclonal Antibodies . | Human Alloantibodies . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | BRIC 108 . | BRIC 224 . | BRIC 221 . | Lub . | Lu5 . | Lu12 . | Lu4 . | Lu8 . | Lu20 . | Lu6 . | Lu7 . | Aub . | Lu13 . |
| Full-length Lu cDNA | 19 | 96 | 114 | 17 | 8 | 6 | 21 | 10 | 12 | 6 | 10 | 11 | 5 |
| Lu-del-5 | 33 | 143 | 196 | 14 | 5 | 4 | 15 | 7 | 7 | 6 | 5 | 1 | 1 |
| Lu-del-4,5 | 17 | 59 | 5.0 | 21 | 8 | 6 | 20 | 11 | 11 | 2 | 1 | 2 | 1 |
| Lu-del-3,4,5 | 17 | 62 | 2.6 | 18 | 8 | 6 | 21 | 9 | 1 | 1 | 2 | 1 | 1 |
| Lu-del-2,3,4,5 | 15 | 227 | 7.4 | 13 | 5 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Untransfected K562 cells (control) | 3.6 | 3.0 | 3.0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 |
| Predicted domain location of antigen/epitope | 1 | 1 | 4 | 1 | 1 | 1 or 2 | 2 | 2 | 3 | 3 or 4 | 4 | 5 | 5 |
| K562 Cells Transfected With cDNA Constructs . | Monoclonal Antibodies . | Human Alloantibodies . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | BRIC 108 . | BRIC 224 . | BRIC 221 . | Lub . | Lu5 . | Lu12 . | Lu4 . | Lu8 . | Lu20 . | Lu6 . | Lu7 . | Aub . | Lu13 . |
| Full-length Lu cDNA | 19 | 96 | 114 | 17 | 8 | 6 | 21 | 10 | 12 | 6 | 10 | 11 | 5 |
| Lu-del-5 | 33 | 143 | 196 | 14 | 5 | 4 | 15 | 7 | 7 | 6 | 5 | 1 | 1 |
| Lu-del-4,5 | 17 | 59 | 5.0 | 21 | 8 | 6 | 20 | 11 | 11 | 2 | 1 | 2 | 1 |
| Lu-del-3,4,5 | 17 | 62 | 2.6 | 18 | 8 | 6 | 21 | 9 | 1 | 1 | 2 | 1 | 1 |
| Lu-del-2,3,4,5 | 15 | 227 | 7.4 | 13 | 5 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Untransfected K562 cells (control) | 3.6 | 3.0 | 3.0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 |
| Predicted domain location of antigen/epitope | 1 | 1 | 4 | 1 | 1 | 1 or 2 | 2 | 2 | 3 | 3 or 4 | 4 | 5 | 5 |
In tests with monoclonal antibodies mean fluorescence intensity (MFI) is given. In tests with human blood group alloantibodies relative mean fluorescence (RMF) calculated as described in Materials and Methods is given.