Table 1. Oligonucleotide Primers Used... | American Society of Hematology

Table 1.

Oligonucleotide Primers Used for Amplifications of Genomic DNA or cDNA Fragments

Location	Forward (5′ to 3′)	Reverse (5′ to 3′)
Genomic DNA primers^*
Exon 8	8F:	8R:
	CAGTTCAATTCTTGTCTTCTTGT	GCTCCAGGACAAAGGCCCT
Exon 9	9F:	9R:
	GGGCCCAACTGTGTCTAAAT	TTATGAGGGGGTGTGGGTT
Exon 10	10F:	10R:
	CAGCGGGTCCACCTTCCT	CCAGCCTCCCGGTCTCT
Exon 11	11F:	11R:
	TTGCCTTAATCACTGTGTCCT	CCTGGGTGTGTGCAACTCT
Exon 12	12F:	12R:
	TTCCTCCATTTTCCCTACTC	CCTGCCTAACATGGTTCTTC
Exon 13	13F:	13R:
	TTCATTCACAACGCCCTGCT	GACCACCCAAAGCATCTGC
Intron 9^†	I9F:
	ACTTGGGATCTGAAGTCACAG
cDNA primers^‡
Exon 7	M7F: TTCTGTCCATGGATTCCAGC
Exon 9	M9F: GTATGCCGTTGTGGGCCTGG
Exon 13		M13R: TTGCTCTGGCGCGTTCTTCC
Exon 14		M14R: GTGATATTGGTGAAGGTAGACGTG

Location	Forward (5′ to 3′)	Reverse (5′ to 3′)
Genomic DNA primers^*
Exon 8	8F:	8R:
	CAGTTCAATTCTTGTCTTCTTGT	GCTCCAGGACAAAGGCCCT
Exon 9	9F:	9R:
	GGGCCCAACTGTGTCTAAAT	TTATGAGGGGGTGTGGGTT
Exon 10	10F:	10R:
	CAGCGGGTCCACCTTCCT	CCAGCCTCCCGGTCTCT
Exon 11	11F:	11R:
	TTGCCTTAATCACTGTGTCCT	CCTGGGTGTGTGCAACTCT
Exon 12	12F:	12R:
	TTCCTCCATTTTCCCTACTC	CCTGCCTAACATGGTTCTTC
Exon 13	13F:	13R:
	TTCATTCACAACGCCCTGCT	GACCACCCAAAGCATCTGC
Intron 9^†	I9F:
	ACTTGGGATCTGAAGTCACAG
cDNA primers^‡
Exon 7	M7F: TTCTGTCCATGGATTCCAGC
Exon 9	M9F: GTATGCCGTTGTGGGCCTGG
Exon 13		M13R: TTGCTCTGGCGCGTTCTTCC
Exon 14		M14R: GTGATATTGGTGAAGGTAGACGTG

Primers for exons are complementary to intronic sequences flanking each exon.

The primer used is about 100 bp upstream to an Alu repeat.

The primers are contained within exon sequences.