Phenotype of Cells Generated by Human CD34+CD38lowCD19− Cord Blood Cells Cultured 6 Weeks at Limiting Dilutions on MS-5 Cells
| Experiment No. . | Condition . | Cells per Well . | No. of Wells Initiated . | No. of Wells With Proliferation . | No. of Wells Positive for . | ||
|---|---|---|---|---|---|---|---|
| . | . | . | . | . | CD19+ Only . | CD11b+ Only . | CD19+ CD11b+ . |
| 1 | Myeloid | 1 | 60 | 11 | 0 | 10 | 0 |
| 10 | 7 | 7 | 0 | 7 | 2 | ||
| Switch | 1 | 60 | 16 | 1 | 6 | 4 | |
| 10 | 7 | 7 | 0 | 0 | 7 | ||
| Lymphoid | 1 | 60 | 10 | 3 | 1 | 5 | |
| 10 | 7 | 7 | 0 | 0 | 7 | ||
| 2 | Lymphoid | 1 | 60 | 14 | 4 | 0 | 3 |
| Switch | 1 | 60 | 4 | 1 | 0 | 3 | |
| 10 | 22 | 22 | 0 | 0 | 22 | ||
| Experiment No. . | Condition . | Cells per Well . | No. of Wells Initiated . | No. of Wells With Proliferation . | No. of Wells Positive for . | ||
|---|---|---|---|---|---|---|---|
| . | . | . | . | . | CD19+ Only . | CD11b+ Only . | CD19+ CD11b+ . |
| 1 | Myeloid | 1 | 60 | 11 | 0 | 10 | 0 |
| 10 | 7 | 7 | 0 | 7 | 2 | ||
| Switch | 1 | 60 | 16 | 1 | 6 | 4 | |
| 10 | 7 | 7 | 0 | 0 | 7 | ||
| Lymphoid | 1 | 60 | 10 | 3 | 1 | 5 | |
| 10 | 7 | 7 | 0 | 0 | 7 | ||
| 2 | Lymphoid | 1 | 60 | 14 | 4 | 0 | 3 |
| Switch | 1 | 60 | 4 | 1 | 0 | 3 | |
| 10 | 22 | 22 | 0 | 0 | 22 | ||
Wells from 96-well plates precoated with the MS-5 cells were seeded with 1 to 50 cord blood cells of the selected phenotype using the automatic cell device unit of the FACS vantage. Cells were cultured in myeloid, switch, or lymphoid conditions as described (see the Materials and Methods). At the end of the 6-week culture period, individual wells were visually inspected and those that contained enough cells were harvested and cells labeled with lineage-specific antibodies. Analysis was performed on the FACSort as described.