Proportion and Phenotype of Human CD19+ B Cells Observed in the Marrow of SCID and NOD-SCID Mice Injected With CB-MNC or CD34+CD19−CD10− Cells
| Experiment No. . | Cells . | Positive/Total Mice . | % Human Cells3-150 . | Mice With CD19+ Cells . | % CD19+3-151 . | % of CD19+ Cells Positive for . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | CD34 . | CD38 . | CD10 . | CD20 . | CD22 . | sIgM . |
| 1 | 34+19−10− | 2/3 | 0.5; 0.23-152 | 2/3 | 40; 90 | 15; 0 | 100 | 100 | 42; 79 | 35; 50 | ND |
| 2 | 34+19−10− | 1/3 | 3.2 | 1/3 | 75 | 21 | 100 | 100 | 36 | 17 | 5 |
| 3 | MNC | 3/3 | 22 ± 9 | 3/3 | 27 ± 1.5 | 10 | 100 | 90 ± 1 | 31 ± 7 | 8 ± 4 | ND |
| 4 | MNC | 3/3 | 39 ± 10 | 3/3 | 64 ± 3 | 8.5 ± 1 | 100 | ND | ND | ND | ND |
| 5 | MNC | 4/4 | 21 ± 6 | 4/4 | 40 ± 15 | ND | 100 | 90 ± 1.2 | 40 ± 4 | 26 ± 2 | 4 ± 1 |
| 6 | MNC | 3/3 | 42 ± 13 | 3/3 | 59 ± 13 | 8 ± 1 | 100 | ND | ND | ND | ND |
| Experiment No. . | Cells . | Positive/Total Mice . | % Human Cells3-150 . | Mice With CD19+ Cells . | % CD19+3-151 . | % of CD19+ Cells Positive for . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | CD34 . | CD38 . | CD10 . | CD20 . | CD22 . | sIgM . |
| 1 | 34+19−10− | 2/3 | 0.5; 0.23-152 | 2/3 | 40; 90 | 15; 0 | 100 | 100 | 42; 79 | 35; 50 | ND |
| 2 | 34+19−10− | 1/3 | 3.2 | 1/3 | 75 | 21 | 100 | 100 | 36 | 17 | 5 |
| 3 | MNC | 3/3 | 22 ± 9 | 3/3 | 27 ± 1.5 | 10 | 100 | 90 ± 1 | 31 ± 7 | 8 ± 4 | ND |
| 4 | MNC | 3/3 | 39 ± 10 | 3/3 | 64 ± 3 | 8.5 ± 1 | 100 | ND | ND | ND | ND |
| 5 | MNC | 4/4 | 21 ± 6 | 4/4 | 40 ± 15 | ND | 100 | 90 ± 1.2 | 40 ± 4 | 26 ± 2 | 4 ± 1 |
| 6 | MNC | 3/3 | 42 ± 13 | 3/3 | 59 ± 13 | 8 ± 1 | 100 | ND | ND | ND | ND |
NOD-SCID (experiments no. 1, 2, 3, and 5) and SCID (experiments no. 4 and 6) mice were injected intravenously with either cord blood light-density MNC (20 × 106 per mouse) or CD34+19−10− cells purified by cell sorting from cord blood CD34+ cells (4 to 6 × 104 per mouse). Mice were killed 4 to 8 weeks later and the proportion and phenotype of human cells in the marrow of each transplanted mouse were examined by labeling the cells with a panel of human lineage-specific markers. Numbers represent the mean (±SD) proportion of cells measured in the indicated number of animals.
Abbreviation: ND, not done.
Human cells were identified by labeling with either anti-CD45 or anti-HLA class I antibodies.
The proportion of CD19+ cells was calculated in a gate including most viable nucleated cells.
Data obtained in both mice are indicated.