CD2 Differentially Affects the Proportions of Thymocyte Subsets in TCR Transgenic Mice
| Mutation . | No. Thymocytes × 10−7 (Mean ± SEM) . | Percent of Total Thymocytes (mean ± SEM) . | |||
|---|---|---|---|---|---|
| . | . | CD4−8− . | CD4+8+ . | CD4+8− . | CD4−8+ . |
| H-Y | 9.8 ± 0.3 | 15 ± 1.7 | 61 ± 3.0 | 8.5 ± 1.0 | 16 ± 1.2 |
| H-Y/CD2o | 9.5 ± 1.5 | 15 ± 1.6 | 46 ± 3.7 | 8.2 ± 0.2 | 31 ± 3.2 |
| 2C | 1.8 ± 0.5 | 51 ± 0.6 | 19 ± 2.0 | 9.4 ± 1.7 | 21 ± 1.8 |
| 2C/CD2o | 1.9 ± 0.4 | 56 ± 2.6 | 13 ± 2.3 | 9.7 ± 1.2 | 21 ± 1.3 |
| Mutation . | No. Thymocytes × 10−7 (Mean ± SEM) . | Percent of Total Thymocytes (mean ± SEM) . | |||
|---|---|---|---|---|---|
| . | . | CD4−8− . | CD4+8+ . | CD4+8− . | CD4−8+ . |
| H-Y | 9.8 ± 0.3 | 15 ± 1.7 | 61 ± 3.0 | 8.5 ± 1.0 | 16 ± 1.2 |
| H-Y/CD2o | 9.5 ± 1.5 | 15 ± 1.6 | 46 ± 3.7 | 8.2 ± 0.2 | 31 ± 3.2 |
| 2C | 1.8 ± 0.5 | 51 ± 0.6 | 19 ± 2.0 | 9.4 ± 1.7 | 21 ± 1.8 |
| 2C/CD2o | 1.9 ± 0.4 | 56 ± 2.6 | 13 ± 2.3 | 9.7 ± 1.2 | 21 ± 1.3 |
Five mice (6 to 12 weeks old) were analyzed per group. Thymocytes from the indicated TCR transgenic mice were stained with phycoerythrin-labeled anti-CD4 and fluorescein isothiocyanate-labeled anti-CD8 MoAbs and analyzed in the FACScan flow cytometer. The total number of viable thymocytes recovered from these transgenic lines are indicated.