IL-10 Inhibits Cell Surface Expression of ICAM-1
| Donor . | IFN-γ . | IL-10 . | Mean Fluorescence Intensity . | |
|---|---|---|---|---|
| . | . | . | ICAM-1 . | HLA-DR . |
| 1 | − | − | 402 (76) | 1,413 (486) |
| − | + | 208 | 319 | |
| + | − | 635 | 3,563 | |
| + | + | 269 | 569 | |
| 2 | − | − | 401 (105) | 1,153 (402) |
| − | + | 262 | 318 | |
| + | − | 928 | 5,044 | |
| + | + | 432 | 940 | |
| 3 | − | − | 996 (160) | 2,023 (1,118) |
| − | + | 578 | 607 | |
| + | − | 2,230 | 9,172 | |
| + | + | 1,277 | 1,544 | |
| 4 | − | − | 913 (181) | 2,858 (869) |
| − | + | 475 | 592 | |
| − | + isotype | 466 | 590 | |
| − | + anti–IL-10 | 740 | 1,739 | |
| + | − | 1,462 | 6,934 | |
| + | + | 938 | 2,593 | |
| + | + isotype | 1,086 | 2,933 | |
| + | + anti–IL-10 | 1,528 | 4,796 | |
| 5 | − | − | 284 (89) | 697 (427) |
| − | + | 214 | 218 | |
| − | + isotype | 212 | 220 | |
| − | + anti–IL-10 | 266 | 605 | |
| + | − | 627 | 2,958 | |
| + | + | 456 | 905 | |
| + | + isotype | 441 | 718 | |
| + | + anti–IL-10 | 597 | 2,410 | |
| Donor . | IFN-γ . | IL-10 . | Mean Fluorescence Intensity . | |
|---|---|---|---|---|
| . | . | . | ICAM-1 . | HLA-DR . |
| 1 | − | − | 402 (76) | 1,413 (486) |
| − | + | 208 | 319 | |
| + | − | 635 | 3,563 | |
| + | + | 269 | 569 | |
| 2 | − | − | 401 (105) | 1,153 (402) |
| − | + | 262 | 318 | |
| + | − | 928 | 5,044 | |
| + | + | 432 | 940 | |
| 3 | − | − | 996 (160) | 2,023 (1,118) |
| − | + | 578 | 607 | |
| + | − | 2,230 | 9,172 | |
| + | + | 1,277 | 1,544 | |
| 4 | − | − | 913 (181) | 2,858 (869) |
| − | + | 475 | 592 | |
| − | + isotype | 466 | 590 | |
| − | + anti–IL-10 | 740 | 1,739 | |
| + | − | 1,462 | 6,934 | |
| + | + | 938 | 2,593 | |
| + | + isotype | 1,086 | 2,933 | |
| + | + anti–IL-10 | 1,528 | 4,796 | |
| 5 | − | − | 284 (89) | 697 (427) |
| − | + | 214 | 218 | |
| − | + isotype | 212 | 220 | |
| − | + anti–IL-10 | 266 | 605 | |
| + | − | 627 | 2,958 | |
| + | + | 456 | 905 | |
| + | + isotype | 441 | 718 | |
| + | + anti–IL-10 | 597 | 2,410 | |
Values in parentheses indicate ICAM-1 and MHC-DR expression on freshly isolated cells given as fluorescence values.