Phenotype of the Lineage Mix Negative, HLA-DR Positive Cells (Before and After Culture) and CMRF-44++ Sorted DC
| Leukocyte . | Lineage Mix Negative Cells . | |||||
|---|---|---|---|---|---|---|
| Differentiation . | Fresh* . | Cultured (phenotypic subpopulations) . | CMRF-44+ Sorted DC . | . | ||
| . | . | . | . | . | (see Fig 4) . | . |
| . | . | . | . | . | . | . |
| Antigen . | HLA-DR+ CMRF-44(+) . | *HLA-DR+ CMRF-44− . | HLA-DR++ CMRF-44+ . | HLA-DR++ CMRF-44++ . | HLA-DR++ CMRF-44++ . | |
| CD83 | − | − | − | + | + | |
| CD40 | − | − | + | ++ | ++ | |
| CD80 | − | − | (+) | + | + | |
| CD86 | − | − | + | ++ | ++ | |
| CD54 | + | + | + | ++ | ++ | |
| CD11b | (+) | (+) | − | + | + | |
| CD13 | (++) | (−/+) | + | + | + | |
| CD14 | − | − | − | − | − | |
| CD33 | (+) | − | + | + | + | |
| CD4 | (+) | (+) | (−/+) | + | + | |
| CD25 | − | (+) | − | + | + | |
| Leukocyte . | Lineage Mix Negative Cells . | |||||
|---|---|---|---|---|---|---|
| Differentiation . | Fresh* . | Cultured (phenotypic subpopulations) . | CMRF-44+ Sorted DC . | . | ||
| . | . | . | . | . | (see Fig 4) . | . |
| . | . | . | . | . | . | . |
| Antigen . | HLA-DR+ CMRF-44(+) . | *HLA-DR+ CMRF-44− . | HLA-DR++ CMRF-44+ . | HLA-DR++ CMRF-44++ . | HLA-DR++ CMRF-44++ . | |
| CD83 | − | − | − | + | + | |
| CD40 | − | − | + | ++ | ++ | |
| CD80 | − | − | (+) | + | + | |
| CD86 | − | − | + | ++ | ++ | |
| CD54 | + | + | + | ++ | ++ | |
| CD11b | (+) | (+) | − | + | + | |
| CD13 | (++) | (−/+) | + | + | + | |
| CD14 | − | − | − | − | − | |
| CD33 | (+) | − | + | + | + | |
| CD4 | (+) | (+) | (−/+) | + | + | |
| CD25 | − | (+) | − | + | + | |
Abbreviations: −, negative; −/+, weakly positive; +, positive; ++, strongly positive; ( ), subpopulation only.
Denotes heterogeneous population.