Ability of Freshly Isolated and CD1a-Enriched Dendritic Cells to Stimulate Periodate-Treated and Allogeneic Lymphocytes
| Patient No. . | Freshly Isolated CML Cells . | CD1a-Selected DCs . | ||
|---|---|---|---|---|
| . | 2,000 Cells . | 1,000 Cells . | 2,000 Cells . | 1,000 Cells . |
| Periodate treated | ||||
| 1 | 642 ± 18 | <100 | 5,561 ± 714 | 2,633 ± 836 |
| 2 | <100 | <100 | 5,705 ± 193 | 3,132 ± 42 |
| 3 | <100 | <100 | 6,251 ± 403 | 3,632 ± 299 |
| 4 | 942 ± 270 | <100 | 14,175 ± 773 | 10,404 ± 118 |
| Allogeneic | ||||
| 1 | 1,924 ± 281 | 879 ± 404 | 13,663 ± 1,092 | 12,107 ± 627 |
| 2 | 1,825 ± 399 | 1,366 ± 114 | 16,131 ± 853 | 12,262 ± 308 |
| 3 | 1,092 ± 439 | 286 ± 523 | 17,430 ± 270 | 15,055 ± 230 |
| 4 | 2,156 ± 351 | 365 ± 311 | 14,150 ± 697 | 15,422 ± 1,610 |
| Patient No. . | Freshly Isolated CML Cells . | CD1a-Selected DCs . | ||
|---|---|---|---|---|
| . | 2,000 Cells . | 1,000 Cells . | 2,000 Cells . | 1,000 Cells . |
| Periodate treated | ||||
| 1 | 642 ± 18 | <100 | 5,561 ± 714 | 2,633 ± 836 |
| 2 | <100 | <100 | 5,705 ± 193 | 3,132 ± 42 |
| 3 | <100 | <100 | 6,251 ± 403 | 3,632 ± 299 |
| 4 | 942 ± 270 | <100 | 14,175 ± 773 | 10,404 ± 118 |
| Allogeneic | ||||
| 1 | 1,924 ± 281 | 879 ± 404 | 13,663 ± 1,092 | 12,107 ± 627 |
| 2 | 1,825 ± 399 | 1,366 ± 114 | 16,131 ± 853 | 12,262 ± 308 |
| 3 | 1,092 ± 439 | 286 ± 523 | 17,430 ± 270 | 15,055 ± 230 |
| 4 | 2,156 ± 351 | 365 ± 311 | 14,150 ± 697 | 15,422 ± 1,610 |
Results represent the mean counts per minute ± SD of triplicate results with 200,000 responder lymphocytes and 2,000 or 1,000 stimulator cells. Counts are corrected for background incorporation of 3H-thymidine by 200,000 responder cells alone.