Colony Formation From Yolk Sac, Fetal Liver, and Adult BM Cells
| Factors . | No. of Colonies . | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Yolk Sac . | Fetal Liver . | BM . | . | . | . | . | . | . | . | . | . | |||||||||
| . | E + Meg . | E . | Meg . | Others . | E + Meg . | E . | Meg . | Others . | E + Meg . | E . | Meg . | Others . | . | . | . | . | . | . | . | . | . |
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 | 18 ± 3 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 12 ± 3 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 9 ± 1 | |||||||||
| EPO | 2 ± 1 | 30 ± 5 | 2 ± 1 | 21 ± 2 | 11 ± 1 | 27 ± 2 | 5 ± 1 | 9 ± 4 | 0 ± 0 | 21 ± 3 | 0 ± 0 | 10 ± 1 | |||||||||
| TPO | 6 ± 2 | 5 ± 1 | 73 ± 5 | 21 ± 2 | 2 ± 1 | 1 ± 1 | 54 ± 5 | 10 ± 2 | 0 ± 0 | 0 ± 0 | 30 ± 1 | 10 ± 1 | |||||||||
| EPO + TPO | 61 ± 3 | 49 ± 4 | 68 ± 7 | 20 ± 3 | 24 ± 2 | 18 ± 1 | 69 ± 6 | 16 ± 2 | 3 ± 1 | 18 ± 1 | 37 ± 1 | 11 ± 2 | |||||||||
| IL-3 + EPO + TPO | 123 ± 7 | 43 ± 5 | 48 ± 5 | 57 ± 9 | 40 ± 2 | 25 ± 6 | 91 ± 1 | 98 ± 1 | 42 ± 4 | 19 ± 2 | 66 ± 9 | 168 ± 9 | |||||||||
| Factors . | No. of Colonies . | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Yolk Sac . | Fetal Liver . | BM . | . | . | . | . | . | . | . | . | . | |||||||||
| . | E + Meg . | E . | Meg . | Others . | E + Meg . | E . | Meg . | Others . | E + Meg . | E . | Meg . | Others . | . | . | . | . | . | . | . | . | . |
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 | 18 ± 3 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 12 ± 3 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 9 ± 1 | |||||||||
| EPO | 2 ± 1 | 30 ± 5 | 2 ± 1 | 21 ± 2 | 11 ± 1 | 27 ± 2 | 5 ± 1 | 9 ± 4 | 0 ± 0 | 21 ± 3 | 0 ± 0 | 10 ± 1 | |||||||||
| TPO | 6 ± 2 | 5 ± 1 | 73 ± 5 | 21 ± 2 | 2 ± 1 | 1 ± 1 | 54 ± 5 | 10 ± 2 | 0 ± 0 | 0 ± 0 | 30 ± 1 | 10 ± 1 | |||||||||
| EPO + TPO | 61 ± 3 | 49 ± 4 | 68 ± 7 | 20 ± 3 | 24 ± 2 | 18 ± 1 | 69 ± 6 | 16 ± 2 | 3 ± 1 | 18 ± 1 | 37 ± 1 | 11 ± 2 | |||||||||
| IL-3 + EPO + TPO | 123 ± 7 | 43 ± 5 | 48 ± 5 | 57 ± 9 | 40 ± 2 | 25 ± 6 | 91 ± 1 | 98 ± 1 | 42 ± 4 | 19 ± 2 | 66 ± 9 | 168 ± 9 | |||||||||
A total of 7.5 × 104 day 10.5 yolk sac cells, 5 × 104 day 13.5 fetal liver cells, and 1 × 105 adult BM cells were plated onto a dish containing 2 U/mL EPO, 10 ng/mL TPO, 100 U/mL IL-3, and their combinations. E + Meg shows the number of colonies that contained both erythroid lineage cells and megakaryocytes. E and Meg shows the number of colonies that contained erythrocytes but no megakaryocytes and those that contained megakaryocytes but no erythrocytes, respectively. Data represent mean ± SE of quadruplicate cultures.