Clonogenic Assays of Fetal BM and Liver Cells Transduced With a Retroviral Vector
| . | CFU-GM . | BFU-E . | % neo Positive Colonies . |
|---|---|---|---|
| Fetal bone marrow no. | |||
| 1 | 56 | 49 | 10 |
| 2 | 53 | 52 | 20 |
| 3 | 47 | 64 | 20 |
| 4 | 74 | 49 | 60 |
| 5 | 106 | 41 | 10 |
| 6 | 35 | 47 | 20 |
| 7 | 35 | 42 | 20 |
| 8 | 27 | 67 | 20 |
| Fetal liver no. | |||
| 1 | 143 | 37 | 30 |
| 2 | 177 | 28 | 10 |
| 3 | 109 | 122 | 0 |
| 4 | 110 | 126 | 20 |
| 5 | 54 | 106 | 20 |
| 6 | 41 | 93 | 10 |
| . | CFU-GM . | BFU-E . | % neo Positive Colonies . |
|---|---|---|---|
| Fetal bone marrow no. | |||
| 1 | 56 | 49 | 10 |
| 2 | 53 | 52 | 20 |
| 3 | 47 | 64 | 20 |
| 4 | 74 | 49 | 60 |
| 5 | 106 | 41 | 10 |
| 6 | 35 | 47 | 20 |
| 7 | 35 | 42 | 20 |
| 8 | 27 | 67 | 20 |
| Fetal liver no. | |||
| 1 | 143 | 37 | 30 |
| 2 | 177 | 28 | 10 |
| 3 | 109 | 122 | 0 |
| 4 | 110 | 126 | 20 |
| 5 | 54 | 106 | 20 |
| 6 | 41 | 93 | 10 |
HFBM or HFL was transduced with a neo containing vector (LN) and then the number of human CFU-GM and BFU-E was measured by clonogenic assays. The % of neo-positive colonies was based on the analysis of 10 randomly picked colonies for incorporated neo gene by PCR analysis with neo-specific primers followed by a Southern blot with an intervening sequence-specific probe.