Colony Formation From Day 6 In Vitro Differentiation-Induced ES Cells
| Factors . | E + Meg . | E . | Meg . | Others . |
|---|---|---|---|---|
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 | 3 ± 1 |
| EPO | 1 ± 1 | 13 ± 2 | 1 ± 1 | 7 ± 2 |
| TPO | 11 ± 23-150 | 2 ± 13-150 | 117 ± 23-150 | 1 ± 1 |
| EPO + TPO | 82 ± 63-151 | 51 ± 23-151 | 37 ± 53-151 | 5 ± 1 |
| IL3 + EPO + TPO | 119 ± 43-152 | 36 ± 5 | 60 ± 43-152 | 17 ± 13-152 |
| Factors . | E + Meg . | E . | Meg . | Others . |
|---|---|---|---|---|
| None | 0 ± 0 | 0 ± 0 | 0 ± 0 | 3 ± 1 |
| EPO | 1 ± 1 | 13 ± 2 | 1 ± 1 | 7 ± 2 |
| TPO | 11 ± 23-150 | 2 ± 13-150 | 117 ± 23-150 | 1 ± 1 |
| EPO + TPO | 82 ± 63-151 | 51 ± 23-151 | 37 ± 53-151 | 5 ± 1 |
| IL3 + EPO + TPO | 119 ± 43-152 | 36 ± 5 | 60 ± 43-152 | 17 ± 13-152 |
A total of 3 × 104 day 6 in vitro differentiation-induced ES cells (for details, Materials and Methods) were plated into a dish containing 2 U/mL EPO, 10 ng/mL TPO, 100 U/mL IL-3, and their combinations. E + Meg, E and Meg shows the numbers of colonies the same as in Table 1. Data represent mean ± SE of quadruplicate cultures.
P < .01 by Student's t-test with the groups of None and TPO alone.
P < .01 by Student's t-test with the group of None, EPO alone, and TPO alone.
P < .05 by Student's t-test with the group of None, EPO alone, TPO alone, and EPO + TPO.