Oligonucleotides and Endonuclease Restriction Enzymes Used to Confirm PK Mutations and Polymorphisms
| Mutation . | Exon . | Primers . | Enzyme . | Size of Normal Fragments (bp) . | Size of Mutated Fragments (bp) . |
|---|---|---|---|---|---|
| IVS3(-2)c | IVS3 | PK4F-4R | AvaI | 211 | 157 + 54 |
| 514C | 6 | PK6F-6R | Hinf I | 231 + 51 + 3 | 282 + 3 |
| 721T | 7 | P721-PK7R | Afl III | 411 | 17 + 394 |
| 787T | 7 | PK7F-PK7R | MspI | 122, 108, 104, 41 | 163, 108, 104 |
| 823A | 7 | PK7F-7R | Eco57I | 375 | 201 + 174 |
| 1168A | 9 | PK9F-9R | Bsr DI | 335 | 250 + 85 |
| 1269C | 9 | PK9F-9R | Aci I | 165 + 150 + 20 | 165 + 135 + 35 |
| 1456T | 11 | PK11F-11R | BsmAI | 220 + 36 | 256 |
| 1529A | 11 | PK11F-11R | Sty I | 256 | 144 + 112 |
| 1552A | 11 | PK11-11R | TspRI | 256 | 141 + 115 |
| 1594T | 11 | PK11F-11R | MspI | 175 + 81 | 256 |
| 1705(A/C) | 12 | PK12F-12R | RcaI | 265 (allele A) | 108 + 157 (allele C) |
| 1738(C/T) | 12 | PK12F-12R | BseRI | 265 (allele C) | 130 + 135 (allele T) |
| Mutation . | Exon . | Primers . | Enzyme . | Size of Normal Fragments (bp) . | Size of Mutated Fragments (bp) . |
|---|---|---|---|---|---|
| IVS3(-2)c | IVS3 | PK4F-4R | AvaI | 211 | 157 + 54 |
| 514C | 6 | PK6F-6R | Hinf I | 231 + 51 + 3 | 282 + 3 |
| 721T | 7 | P721-PK7R | Afl III | 411 | 17 + 394 |
| 787T | 7 | PK7F-PK7R | MspI | 122, 108, 104, 41 | 163, 108, 104 |
| 823A | 7 | PK7F-7R | Eco57I | 375 | 201 + 174 |
| 1168A | 9 | PK9F-9R | Bsr DI | 335 | 250 + 85 |
| 1269C | 9 | PK9F-9R | Aci I | 165 + 150 + 20 | 165 + 135 + 35 |
| 1456T | 11 | PK11F-11R | BsmAI | 220 + 36 | 256 |
| 1529A | 11 | PK11F-11R | Sty I | 256 | 144 + 112 |
| 1552A | 11 | PK11-11R | TspRI | 256 | 141 + 115 |
| 1594T | 11 | PK11F-11R | MspI | 175 + 81 | 256 |
| 1705(A/C) | 12 | PK12F-12R | RcaI | 265 (allele A) | 108 + 157 (allele C) |
| 1738(C/T) | 12 | PK12F-12R | BseRI | 265 (allele C) | 130 + 135 (allele T) |