Specific Lysis of Various Target Cells by 9 Clones
| Target . | HLA . | % Lysis by Clones . | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | A . | B . | DRB1* . | DQB1* . | CD8+ . | CD4+ . | . | . | . | . | . | . | . | |||||||
| . | . | . | . | . | 12 . | 28 . | 35 . | 41 . | 13 . | 65 . | 67 . | 69 . | 70 . | . | . | . | . | . | . | . |
| 1. | *0201, 2402 | *3501, 5101 | 1502, 08032 | 0601 | 0 | 0 | 0 | 0 | 23.0 | 0 | 0 | 30.3 | 24.0 | |||||||
| 2. | 11, 19 | 7, 40 | 1001, 1100 | 0501, 0301 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||||||
| 3. | 24 | 52 | 2 | 3 | 45.8 | NT | NT | NT | 0 | 0 | 0 | 0 | 0 | |||||||
| 4. | *2402, 3303 | *4403, 5201 | 1502, 1406 | 0301, 0601 | 29.1 | 31.9 | 53.2 | 33.8 | 24.3 | 0 | 0 | 41.0 | 18.3 | |||||||
| 5. | 1 | 52 | 0101 | 0501 | 76.6 | 46.5 | 87.8 | 88.3 | 0 | 0 | 0 | 0 | 0 | |||||||
| 6. | *2402, 2602 | *5201, 4002 | 1405, 0901 | 03032, 05031 | NT | 26.2 | 44.8 | 15.7 | 0 | 0 | 0 | 0 | 0 | |||||||
| 7. | *2402, 1101 | *5201, 1501 | 0406, 1501 | 0302, 0602 | NT | 22.6 | 29.5 | 20.5 | 0 | 0 | 0 | 34.9 | 4.2 | |||||||
| 8. | *2402, 3303 | *5201, 4403 | 1302, 1501 | 0602, 0604 | NT | NT | NT | NT | 2.6 | 0 | 0 | 16.7 | 0 | |||||||
| Target . | HLA . | % Lysis by Clones . | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | A . | B . | DRB1* . | DQB1* . | CD8+ . | CD4+ . | . | . | . | . | . | . | . | |||||||
| . | . | . | . | . | 12 . | 28 . | 35 . | 41 . | 13 . | 65 . | 67 . | 69 . | 70 . | . | . | . | . | . | . | . |
| 1. | *0201, 2402 | *3501, 5101 | 1502, 08032 | 0601 | 0 | 0 | 0 | 0 | 23.0 | 0 | 0 | 30.3 | 24.0 | |||||||
| 2. | 11, 19 | 7, 40 | 1001, 1100 | 0501, 0301 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||||||
| 3. | 24 | 52 | 2 | 3 | 45.8 | NT | NT | NT | 0 | 0 | 0 | 0 | 0 | |||||||
| 4. | *2402, 3303 | *4403, 5201 | 1502, 1406 | 0301, 0601 | 29.1 | 31.9 | 53.2 | 33.8 | 24.3 | 0 | 0 | 41.0 | 18.3 | |||||||
| 5. | 1 | 52 | 0101 | 0501 | 76.6 | 46.5 | 87.8 | 88.3 | 0 | 0 | 0 | 0 | 0 | |||||||
| 6. | *2402, 2602 | *5201, 4002 | 1405, 0901 | 03032, 05031 | NT | 26.2 | 44.8 | 15.7 | 0 | 0 | 0 | 0 | 0 | |||||||
| 7. | *2402, 1101 | *5201, 1501 | 0406, 1501 | 0302, 0602 | NT | 22.6 | 29.5 | 20.5 | 0 | 0 | 0 | 34.9 | 4.2 | |||||||
| 8. | *2402, 3303 | *5201, 4403 | 1302, 1501 | 0602, 0604 | NT | NT | NT | NT | 2.6 | 0 | 0 | 16.7 | 0 | |||||||
Specific lysis of various B-LCL targets by 9 clones was measured using a standard 4-hour 51Cr release assay at an E:T ratio of 2:1. HLAs of targets and the percentage of specific lysis by each clone are indicated. Greater than 10% specific lysis was scored as positive lysis and <10% specific lysis was scored as negative lysis. Donor HLA: A24, B38/61, DRB1*0901; patient HLA: A*2402, B*4002/52011, DRB1*1502/0901.
Abbreviation: NT, not tested.