Table 1. Phenotype, Genotype,... | American Society of Hematology

Table 1.

Phenotype, Genotype, Proto-Oncogene Configuration, and Suppressor Gene Mutation of Leukemic Cells in Five IgA and/or IgG CLLs

Phenotypic Markers cDNA Probes and Primers	Restriction Enzymes	Patients
		TR %	KA %	GR %	TE %	SC %
IgM		10	0	0	0	4
IgD		8	0	0	0	0
IgA1		65	77	84	90	0
IgA2		2	0	0	0	0
IgG1		0	0	0	2	0
IgG2		0	0	0	0	0
IgG3		6	0	4	90	55
IgG4		2	0	0	0	0
κ		62	0	86	90	43
λ		15	75	0	2	8
HLA-DR		66	95	89	88	86
CD10		2	0	0	0	0
CD19		66	86	87	90	65
CD20		61	89	86	92	60
CD23		64	90	86	92	58
CD3		19	5	4	7	15
CD5		61	89	87	91	60
Cyclin D1		0	0	0	0	0
J_H	EcoRI	R (1)	R (1)	R (2)	R (1)	R (1)
J_H	BamHI	R (2)	R (1)	R (2)	R (1)	R (1)
C_μ	BamHI	G	G	D	G	G
bcl-1 (MTC)	HindIII	G	G	G	G	G
bcl-1 (p94PS)	EcoRI	G	G	G	G	G
bcl-2 (pFL-1)	BamHI	G	G	G	G	G
bcl-2 (pFL-2)	BamHI	G	G	G	G	G
bcl-2 (pB16)	BamHI	G	G	G	G	G
p53 exon 5		WT	WT	WT	WT	WT
p53 exon 6		WT	WT	WT	WT	WT
p53 exon 7		WT	WT	WT	WT	WT
p53 exon 8		WT	WT	WT	WT	WT
p53 exon 9		WT	WT	WT	WT	WT

Phenotypic Markers cDNA Probes and Primers	Restriction Enzymes	Patients
		TR %	KA %	GR %	TE %	SC %
IgM		10	0	0	0	4
IgD		8	0	0	0	0
IgA1		65	77	84	90	0
IgA2		2	0	0	0	0
IgG1		0	0	0	2	0
IgG2		0	0	0	0	0
IgG3		6	0	4	90	55
IgG4		2	0	0	0	0
κ		62	0	86	90	43
λ		15	75	0	2	8
HLA-DR		66	95	89	88	86
CD10		2	0	0	0	0
CD19		66	86	87	90	65
CD20		61	89	86	92	60
CD23		64	90	86	92	58
CD3		19	5	4	7	15
CD5		61	89	87	91	60
Cyclin D1		0	0	0	0	0
J_H	EcoRI	R (1)	R (1)	R (2)	R (1)	R (1)
J_H	BamHI	R (2)	R (1)	R (2)	R (1)	R (1)
C_μ	BamHI	G	G	D	G	G
bcl-1 (MTC)	HindIII	G	G	G	G	G
bcl-1 (p94PS)	EcoRI	G	G	G	G	G
bcl-2 (pFL-1)	BamHI	G	G	G	G	G
bcl-2 (pFL-2)	BamHI	G	G	G	G	G
bcl-2 (pB16)	BamHI	G	G	G	G	G
p53 exon 5		WT	WT	WT	WT	WT
p53 exon 6		WT	WT	WT	WT	WT
p53 exon 7		WT	WT	WT	WT	WT
p53 exon 8		WT	WT	WT	WT	WT
p53 exon 9		WT	WT	WT	WT	WT

Abbreviations: D, deleted; G, germline; R (1-2), rearranged (number of rearranged bands); WT, wild type.